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Dynamic, size-selective effects of protamine sulfate and hyaluronidase on the rat glomerular filtration barrier in vivo.

Sverrisson, Kristinn LU ; Axelsson, Josefin LU ; Rippe, Anna LU ; Asgeirsson, Daniel LU and Rippe, Bengt LU (2014) In American Journal of Physiology-Renal Physiology 307(10). p.1136-1143
Abstract
The proteinuric actions of protamine sulfate (PS) have classically been, at least partly, attributed to alterations of the negatively charged glomerular endothelial glycocalyx (GC). To investigate whether the charge-selective properties of the glomerular filtration barrier (GFB) would be altered by PS we assessed the glomerular sieving of conventional, uncharged, polydispersed Ficoll (n-Ficoll) compared to charge modified, conformationally intact, anionic (carboxymethylated) Ficoll (a-Ficoll) before and after systemic infusions of PS in rats. For comparison we also investigated the impact of hyaluronidase (Hyase), which partially degrades the GC, on GFB permeability. In anaesthetized Wistar rats blood access was achieved and the left... (More)
The proteinuric actions of protamine sulfate (PS) have classically been, at least partly, attributed to alterations of the negatively charged glomerular endothelial glycocalyx (GC). To investigate whether the charge-selective properties of the glomerular filtration barrier (GFB) would be altered by PS we assessed the glomerular sieving of conventional, uncharged, polydispersed Ficoll (n-Ficoll) compared to charge modified, conformationally intact, anionic (carboxymethylated) Ficoll (a-Ficoll) before and after systemic infusions of PS in rats. For comparison we also investigated the impact of hyaluronidase (Hyase), which partially degrades the GC, on GFB permeability. In anaesthetized Wistar rats blood access was achieved and the left ureter was cannulated for urine collection. Rats were infused with either n-Ficoll or a-Ficoll before and during systemic infusions with either PS or Hyase. Plasma and urine samples were taken repeatedly and analyzed by high performance size exclusion chromatography (HPSEC) for assessing glomerular sieving coefficients (θ) for Ficoll (radius 10-80Å). The GFB showed a significant glomerular charge selectivity for Ficoll molecules of radius 20-35Å (Ficoll20-35Å). PS and Hyase infusions reversibly increased θ for large Ficoll molecules (Ficoll50-80Å). Thus, for PS θ for a-Ficoll70Å increased from 2.47 x 10(-5) ± 1.1(-5) to 7.25 x 10(-5) ± 1.1(-5) (P<0.05) at 15 min. For Hyase the changes in a-Ficoll50-80Å were, however, not statistically significant. Neither PS nor Hyase had any effect on θ for n-Ficoll20-45Å or a-Ficoll20-45Å. It is concluded that systemically administered PS and Hyase in moderate doses dynamically decreased the size-selectivity of the rat GFB without affecting its charge selective properties. (Less)
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type
Contribution to journal
publication status
published
subject
in
American Journal of Physiology-Renal Physiology
volume
307
issue
10
pages
1136 - 1143
publisher
American Physiological Society
external identifiers
  • pmid:25209861
  • wos:000345250600006
  • scopus:84924052712
ISSN
1522-1466
DOI
10.1152/ajprenal.00181.2014
language
English
LU publication?
yes
id
f7589a52-0dbc-4563-b0f4-a2fc6667ee5e (old id 4691870)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25209861?dopt=Abstract
date added to LUP
2014-10-05 19:59:30
date last changed
2017-09-03 03:04:41
@article{f7589a52-0dbc-4563-b0f4-a2fc6667ee5e,
  abstract     = {The proteinuric actions of protamine sulfate (PS) have classically been, at least partly, attributed to alterations of the negatively charged glomerular endothelial glycocalyx (GC). To investigate whether the charge-selective properties of the glomerular filtration barrier (GFB) would be altered by PS we assessed the glomerular sieving of conventional, uncharged, polydispersed Ficoll (n-Ficoll) compared to charge modified, conformationally intact, anionic (carboxymethylated) Ficoll (a-Ficoll) before and after systemic infusions of PS in rats. For comparison we also investigated the impact of hyaluronidase (Hyase), which partially degrades the GC, on GFB permeability. In anaesthetized Wistar rats blood access was achieved and the left ureter was cannulated for urine collection. Rats were infused with either n-Ficoll or a-Ficoll before and during systemic infusions with either PS or Hyase. Plasma and urine samples were taken repeatedly and analyzed by high performance size exclusion chromatography (HPSEC) for assessing glomerular sieving coefficients (θ) for Ficoll (radius 10-80Å). The GFB showed a significant glomerular charge selectivity for Ficoll molecules of radius 20-35Å (Ficoll20-35Å). PS and Hyase infusions reversibly increased θ for large Ficoll molecules (Ficoll50-80Å). Thus, for PS θ for a-Ficoll70Å increased from 2.47 x 10(-5) ± 1.1(-5) to 7.25 x 10(-5) ± 1.1(-5) (P&lt;0.05) at 15 min. For Hyase the changes in a-Ficoll50-80Å were, however, not statistically significant. Neither PS nor Hyase had any effect on θ for n-Ficoll20-45Å or a-Ficoll20-45Å. It is concluded that systemically administered PS and Hyase in moderate doses dynamically decreased the size-selectivity of the rat GFB without affecting its charge selective properties.},
  author       = {Sverrisson, Kristinn and Axelsson, Josefin and Rippe, Anna and Asgeirsson, Daniel and Rippe, Bengt},
  issn         = {1522-1466},
  language     = {eng},
  number       = {10},
  pages        = {1136--1143},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology-Renal Physiology},
  title        = {Dynamic, size-selective effects of protamine sulfate and hyaluronidase on the rat glomerular filtration barrier in vivo.},
  url          = {http://dx.doi.org/10.1152/ajprenal.00181.2014},
  volume       = {307},
  year         = {2014},
}