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Quantitative proteomics at different depths in human articular cartilage reveals unique patterns of protein distribution.

Muller, Catharina LU ; Khabut, Areej LU ; Dudhia, Jayesh; Reinholt, Finn P; Aspberg, Anders LU ; Heinegård, Dick LU and Önnerfjord, Patrik LU (2014) In Matrix Biology 40(Sep 1). p.34-45
Abstract
The articular cartilage of synovial joints ensures friction-free mobility and attenuates mechanical impact on the joint during movement. These functions are mediated by the complex network of extracellular molecules characteristic for articular cartilage. Zonal differences in the extracellular matrix (ECM) are well recognized. However, knowledge about the precise molecular composition in the different zones remains limited. In the present study, we investigated the distribution of ECM molecules along the surface-to-bone axis, using quantitative non-targeted as well as targeted proteomics.\ In a discovery approach, iTRAQ mass spectrometry was used to identify all extractable ECM proteins in the different layers of a human lateral tibial... (More)
The articular cartilage of synovial joints ensures friction-free mobility and attenuates mechanical impact on the joint during movement. These functions are mediated by the complex network of extracellular molecules characteristic for articular cartilage. Zonal differences in the extracellular matrix (ECM) are well recognized. However, knowledge about the precise molecular composition in the different zones remains limited. In the present study, we investigated the distribution of ECM molecules along the surface-to-bone axis, using quantitative non-targeted as well as targeted proteomics.\ In a discovery approach, iTRAQ mass spectrometry was used to identify all extractable ECM proteins in the different layers of a human lateral tibial plateau full thickness cartilage sample. A targeted MRM mass spectrometry approach was then applied to verify these findings and to extend the analysis to four medial tibial plateau samples. In the lateral tibial plateau sample, the unique distribution patterns of 70 ECM proteins were identified, revealing groups of proteins with a preferential distribution to the superficial, intermediate or deep regions of articular cartilage. The detailed analysis of selected 29 proteins confirmed these findings and revealed similar distribution patterns in the four medial tibial plateau samples. The results of this study allow, for the first time, an overview of the zonal distribution of a broad range of cartilage ECM proteins and open up further investigations of the functional roles of matrix proteins in the different zones of articular cartilage in health and disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Matrix Biology
volume
40
issue
Sep 1
pages
34 - 45
publisher
Elsevier
external identifiers
  • pmid:25193283
  • wos:000347597700006
  • scopus:84918514581
ISSN
1569-1802
DOI
10.1016/j.matbio.2014.08.013
language
English
LU publication?
yes
id
0a4e16a8-6092-44af-891a-0f969ca8655d (old id 4692210)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25193283?dopt=Abstract
date added to LUP
2014-10-02 19:34:07
date last changed
2017-10-22 03:27:07
@article{0a4e16a8-6092-44af-891a-0f969ca8655d,
  abstract     = {The articular cartilage of synovial joints ensures friction-free mobility and attenuates mechanical impact on the joint during movement. These functions are mediated by the complex network of extracellular molecules characteristic for articular cartilage. Zonal differences in the extracellular matrix (ECM) are well recognized. However, knowledge about the precise molecular composition in the different zones remains limited. In the present study, we investigated the distribution of ECM molecules along the surface-to-bone axis, using quantitative non-targeted as well as targeted proteomics.\ In a discovery approach, iTRAQ mass spectrometry was used to identify all extractable ECM proteins in the different layers of a human lateral tibial plateau full thickness cartilage sample. A targeted MRM mass spectrometry approach was then applied to verify these findings and to extend the analysis to four medial tibial plateau samples. In the lateral tibial plateau sample, the unique distribution patterns of 70 ECM proteins were identified, revealing groups of proteins with a preferential distribution to the superficial, intermediate or deep regions of articular cartilage. The detailed analysis of selected 29 proteins confirmed these findings and revealed similar distribution patterns in the four medial tibial plateau samples. The results of this study allow, for the first time, an overview of the zonal distribution of a broad range of cartilage ECM proteins and open up further investigations of the functional roles of matrix proteins in the different zones of articular cartilage in health and disease.},
  author       = {Muller, Catharina and Khabut, Areej and Dudhia, Jayesh and Reinholt, Finn P and Aspberg, Anders and Heinegård, Dick and Önnerfjord, Patrik},
  issn         = {1569-1802},
  language     = {eng},
  number       = {Sep 1},
  pages        = {34--45},
  publisher    = {Elsevier},
  series       = {Matrix Biology},
  title        = {Quantitative proteomics at different depths in human articular cartilage reveals unique patterns of protein distribution.},
  url          = {http://dx.doi.org/10.1016/j.matbio.2014.08.013},
  volume       = {40},
  year         = {2014},
}