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Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines

Svensson, Daniel LU ; Aidoukovitch, Alexandra LU ; Anders, Emma ; Jönsson, Daniel LU ; Nebel, Daniel LU and Nilsson, Bengt Olof LU orcid (2017) In Inflammation Research 66(9). p.823-831
Abstract

Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular... (More)

Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular expression level of SLPI negatively correlated to LPS-induced stimulation of IL-6 and MCP-1. Both SLPI gene activity and protein were reduced by about 70% in PDL cells treated with SLPI siRNA compared to cells treated with non-coding construct. Treatment with SLPI siRNA was associated with up-regulation of both basal and LPS-stimulated IL-6, MCP-1 and TLRs mRNA expression. The up-regulation of MCP-1 transcript in SLPI siRNA-treated cells was confirmed on protein level. SLPI siRNA-treatment enhanced the phosphorylated NF-κB p105 protein expression. Conclusions: SLPI regulates PDL cell pro-inflammatory cytokine expression and modulates NF-κB signaling, suggesting that SLPI governs the immune cell-like properties of PDL cells.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytokines, NF-κB, Periodontal ligament cells, Periodontitis, Secretory leukocyte protease inhibitor
in
Inflammation Research
volume
66
issue
9
pages
823 - 831
publisher
Birkhäuser Verlag
external identifiers
  • scopus:85020420295
  • wos:000406415700009
  • pmid:28597116
ISSN
1023-3830
DOI
10.1007/s00011-017-1062-2
project
Production and transport of the antimicrobial peptide LL-37 and other peptides in saliva: physiological and pathophysiological importance
language
English
LU publication?
yes
id
47161121-beeb-4c66-a6c6-e40cb03e15bb
date added to LUP
2017-06-26 16:16:21
date last changed
2022-09-22 22:07:14
@article{47161121-beeb-4c66-a6c6-e40cb03e15bb,
  abstract     = {{<p>Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular expression level of SLPI negatively correlated to LPS-induced stimulation of IL-6 and MCP-1. Both SLPI gene activity and protein were reduced by about 70% in PDL cells treated with SLPI siRNA compared to cells treated with non-coding construct. Treatment with SLPI siRNA was associated with up-regulation of both basal and LPS-stimulated IL-6, MCP-1 and TLRs mRNA expression. The up-regulation of MCP-1 transcript in SLPI siRNA-treated cells was confirmed on protein level. SLPI siRNA-treatment enhanced the phosphorylated NF-κB p105 protein expression. Conclusions: SLPI regulates PDL cell pro-inflammatory cytokine expression and modulates NF-κB signaling, suggesting that SLPI governs the immune cell-like properties of PDL cells.</p>}},
  author       = {{Svensson, Daniel and Aidoukovitch, Alexandra and Anders, Emma and Jönsson, Daniel and Nebel, Daniel and Nilsson, Bengt Olof}},
  issn         = {{1023-3830}},
  keywords     = {{Cytokines; NF-κB; Periodontal ligament cells; Periodontitis; Secretory leukocyte protease inhibitor}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{9}},
  pages        = {{823--831}},
  publisher    = {{Birkhäuser Verlag}},
  series       = {{Inflammation Research}},
  title        = {{Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines}},
  url          = {{http://dx.doi.org/10.1007/s00011-017-1062-2}},
  doi          = {{10.1007/s00011-017-1062-2}},
  volume       = {{66}},
  year         = {{2017}},
}