Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines
(2017) In Inflammation Research 66(9). p.823-831- Abstract
Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular... (More)
Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular expression level of SLPI negatively correlated to LPS-induced stimulation of IL-6 and MCP-1. Both SLPI gene activity and protein were reduced by about 70% in PDL cells treated with SLPI siRNA compared to cells treated with non-coding construct. Treatment with SLPI siRNA was associated with up-regulation of both basal and LPS-stimulated IL-6, MCP-1 and TLRs mRNA expression. The up-regulation of MCP-1 transcript in SLPI siRNA-treated cells was confirmed on protein level. SLPI siRNA-treatment enhanced the phosphorylated NF-κB p105 protein expression. Conclusions: SLPI regulates PDL cell pro-inflammatory cytokine expression and modulates NF-κB signaling, suggesting that SLPI governs the immune cell-like properties of PDL cells.
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- author
- Svensson, Daniel LU ; Aidoukovitch, Alexandra LU ; Anders, Emma ; Jönsson, Daniel LU ; Nebel, Daniel LU and Nilsson, Bengt Olof LU
- organization
- publishing date
- 2017-06-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cytokines, NF-κB, Periodontal ligament cells, Periodontitis, Secretory leukocyte protease inhibitor
- in
- Inflammation Research
- volume
- 66
- issue
- 9
- pages
- 823 - 831
- publisher
- Birkhäuser
- external identifiers
-
- wos:000406415700009
- pmid:28597116
- scopus:85020420295
- ISSN
- 1023-3830
- DOI
- 10.1007/s00011-017-1062-2
- project
- Production and transport of the antimicrobial peptide LL-37 and other peptides in saliva: physiological and pathophysiological importance
- language
- English
- LU publication?
- yes
- id
- 47161121-beeb-4c66-a6c6-e40cb03e15bb
- date added to LUP
- 2017-06-26 16:16:21
- date last changed
- 2024-08-04 23:56:33
@article{47161121-beeb-4c66-a6c6-e40cb03e15bb, abstract = {{<p>Objective: Regulation of immune-like cell properties of periodontal ligament (PDL) cells is not understood. We investigate the importance of secretory leukocyte protease inhibitor (SLPI) for production of pro-inflammatory cytokines in human PDL cells. Materials and methods: PDL cells were isolated from teeth extracted for orthodontic reasons. Cellular location of SLPI was investigated by immunocytochemistry. Cytokine transcript and protein expression were assessed by quantitative real-time RT-PCR and Western blotting. SLPI gene activity was knocked-down by siRNA. NF-κB signaling was assessed by measuring IκBα, and phosphorylated p65 and p105 protein expression. Results: PDL cells showed cytoplasmic expression of SLPI. Cellular expression level of SLPI negatively correlated to LPS-induced stimulation of IL-6 and MCP-1. Both SLPI gene activity and protein were reduced by about 70% in PDL cells treated with SLPI siRNA compared to cells treated with non-coding construct. Treatment with SLPI siRNA was associated with up-regulation of both basal and LPS-stimulated IL-6, MCP-1 and TLRs mRNA expression. The up-regulation of MCP-1 transcript in SLPI siRNA-treated cells was confirmed on protein level. SLPI siRNA-treatment enhanced the phosphorylated NF-κB p105 protein expression. Conclusions: SLPI regulates PDL cell pro-inflammatory cytokine expression and modulates NF-κB signaling, suggesting that SLPI governs the immune cell-like properties of PDL cells.</p>}}, author = {{Svensson, Daniel and Aidoukovitch, Alexandra and Anders, Emma and Jönsson, Daniel and Nebel, Daniel and Nilsson, Bengt Olof}}, issn = {{1023-3830}}, keywords = {{Cytokines; NF-κB; Periodontal ligament cells; Periodontitis; Secretory leukocyte protease inhibitor}}, language = {{eng}}, month = {{06}}, number = {{9}}, pages = {{823--831}}, publisher = {{Birkhäuser}}, series = {{Inflammation Research}}, title = {{Secretory leukocyte protease inhibitor regulates human periodontal ligament cell production of pro-inflammatory cytokines}}, url = {{http://dx.doi.org/10.1007/s00011-017-1062-2}}, doi = {{10.1007/s00011-017-1062-2}}, volume = {{66}}, year = {{2017}}, }