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Establishment and characterization of a human papillomavirus type 16-positive tonsillar carcinoma xenograft in BALB/c nude mice

Letsolo, Boitelo T LU ; Faust, Helena LU ; Ekblad, Lars LU ; Wennerberg, Johan LU and Forslund, Ola LU (2016) In Head and Neck 38(3). p.25-417
Abstract

BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.

METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).

RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in... (More)

BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.

METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).

RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in 14 xenografts from the second passage. From this passage, a viral load of 6.4 copies/cell (range = 4.6-9.6) and 3.7 (range = 1.0-5.5) E7-mRNA transcripts/HPV16-genome were detected.

CONCLUSION: This xenograft represents the first established HPV16-positive tonsillar tumor in nude mice and could provide an experimental system of HPV16-positive tonsillar cancers. © 2015 Wiley Periodicals, Inc. Head Neck 38: 417-425, 2016.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Head and Neck
volume
38
issue
3
pages
9 pages
publisher
John Wiley & Sons
external identifiers
  • pmid:25352201
  • scopus:84958212706
  • wos:000370187400017
ISSN
1043-3074
DOI
10.1002/hed.23918
language
English
LU publication?
yes
id
6a8a4d3b-5a81-4d10-80d5-3595d8fee24c (old id 4732991)
date added to LUP
2014-11-10 20:30:19
date last changed
2017-08-24 14:32:36
@article{6a8a4d3b-5a81-4d10-80d5-3595d8fee24c,
  abstract     = {<p>BACKGROUND: Among head and neck cancers, human papillomavirus type 16 (HPV16) is associated with tonsillar carcinomas. Despite this, no HPV16-positive tonsillar cancer cell line has been established in nude mice.</p><p>METHODS: Fresh tonsillar carcinoma biopsies were obtained from 23 patients and implanted subcutaneously into nude mice (BALB/c, nu/nu).</p><p>RESULTS: After 7 months, one xenograft was established. The primary tumor harbored 2.7 copies (95% confidence interval = 2.4-2.9) of HPV16/cell and displayed 99.9% (7904/7906) nucleotide identity to HPV16 (EU118173.1). The xenograft showed increased methylation in two E2-binding sites of the HPV16 genome. Both episomal and integrated HPV16 were detected in the original tumor and in 14 xenografts from the second passage. From this passage, a viral load of 6.4 copies/cell (range = 4.6-9.6) and 3.7 (range = 1.0-5.5) E7-mRNA transcripts/HPV16-genome were detected.</p><p>CONCLUSION: This xenograft represents the first established HPV16-positive tonsillar tumor in nude mice and could provide an experimental system of HPV16-positive tonsillar cancers. © 2015 Wiley Periodicals, Inc. Head Neck 38: 417-425, 2016.</p>},
  author       = {Letsolo, Boitelo T and Faust, Helena and Ekblad, Lars and Wennerberg, Johan and Forslund, Ola},
  issn         = {1043-3074},
  language     = {eng},
  number       = {3},
  pages        = {25--417},
  publisher    = {John Wiley & Sons},
  series       = {Head and Neck},
  title        = {Establishment and characterization of a human papillomavirus type 16-positive tonsillar carcinoma xenograft in BALB/c nude mice},
  url          = {http://dx.doi.org/10.1002/hed.23918},
  volume       = {38},
  year         = {2016},
}