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Interactions of PAMAM Dendrimers with Negatively Charged Model Biomembranes.

Yanez, Marianna LU ; Ainalem, Marie-Louise LU ; Porcar, Lionel; Martel, Anne; Coker, Helena; Lundberg, Dan LU ; Chang, Debby P; Soltwedel, Olaf; Barker, Robert and Nylander, Tommy LU (2014) In The Journal of Physical Chemistry Part B 118(45). p.12892-12906
Abstract
We have investigated the interactions between cationic poly(amidoamine) (PAMAM) dendrimers of generation 4 (G4), a potential gene transfection vector, with net-anionic model biomembranes composed of different ratios of zwitterionic phosphocholine (PC) and anionic phospho-l-serine (PS) phospholipids. Two types of model membranes were used: solid-supported bilayers, prepared with lipids carrying palmitoyl-oleoyl (PO) and diphytanoyl (DPh) acyl chains, and free-standing bilayers, formed at the interface between two aqueous droplets in oil (droplet interface bilayers, DIBs) using the DPh-based lipids. G4 dendrimers were found to translocate through POPC:POPS bilayers deposited on silica surfaces. The charge density of the bilayer affects... (More)
We have investigated the interactions between cationic poly(amidoamine) (PAMAM) dendrimers of generation 4 (G4), a potential gene transfection vector, with net-anionic model biomembranes composed of different ratios of zwitterionic phosphocholine (PC) and anionic phospho-l-serine (PS) phospholipids. Two types of model membranes were used: solid-supported bilayers, prepared with lipids carrying palmitoyl-oleoyl (PO) and diphytanoyl (DPh) acyl chains, and free-standing bilayers, formed at the interface between two aqueous droplets in oil (droplet interface bilayers, DIBs) using the DPh-based lipids. G4 dendrimers were found to translocate through POPC:POPS bilayers deposited on silica surfaces. The charge density of the bilayer affects translocation, which is reduced when the ionic strength increases. This shows that the dendrimer-bilayer interactions are largely controlled by their electrostatic attraction. The structure of the solid-supported bilayers remains intact upon translocation of the dendrimer. However, the amount of lipids in the bilayer decreases and dendrimer/lipid aggregates are formed in bulk solution, which can be deposited on the interfacial layers upon dilution of the system with dendrimer-free solvent. Electrophysiology measurements on DIBs confirm that G4 dendrimers cross the lipid membranes containing PS, which then become more permeable to ions. The obtained results have implications for PAMAM dendrimers as delivery vehicles to cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
118
issue
45
pages
12892 - 12906
publisher
The American Chemical Society
external identifiers
  • wos:000344976600010
  • pmid:25310456
  • scopus:84922640545
ISSN
1520-5207
DOI
10.1021/jp506510s
language
English
LU publication?
yes
id
f6ef0f9c-b4f3-4878-b3cf-991a1f59b786 (old id 4737016)
date added to LUP
2014-11-17 11:34:51
date last changed
2017-11-12 03:04:49
@article{f6ef0f9c-b4f3-4878-b3cf-991a1f59b786,
  abstract     = {We have investigated the interactions between cationic poly(amidoamine) (PAMAM) dendrimers of generation 4 (G4), a potential gene transfection vector, with net-anionic model biomembranes composed of different ratios of zwitterionic phosphocholine (PC) and anionic phospho-l-serine (PS) phospholipids. Two types of model membranes were used: solid-supported bilayers, prepared with lipids carrying palmitoyl-oleoyl (PO) and diphytanoyl (DPh) acyl chains, and free-standing bilayers, formed at the interface between two aqueous droplets in oil (droplet interface bilayers, DIBs) using the DPh-based lipids. G4 dendrimers were found to translocate through POPC:POPS bilayers deposited on silica surfaces. The charge density of the bilayer affects translocation, which is reduced when the ionic strength increases. This shows that the dendrimer-bilayer interactions are largely controlled by their electrostatic attraction. The structure of the solid-supported bilayers remains intact upon translocation of the dendrimer. However, the amount of lipids in the bilayer decreases and dendrimer/lipid aggregates are formed in bulk solution, which can be deposited on the interfacial layers upon dilution of the system with dendrimer-free solvent. Electrophysiology measurements on DIBs confirm that G4 dendrimers cross the lipid membranes containing PS, which then become more permeable to ions. The obtained results have implications for PAMAM dendrimers as delivery vehicles to cells.},
  author       = {Yanez, Marianna and Ainalem, Marie-Louise and Porcar, Lionel and Martel, Anne and Coker, Helena and Lundberg, Dan and Chang, Debby P and Soltwedel, Olaf and Barker, Robert and Nylander, Tommy},
  issn         = {1520-5207},
  language     = {eng},
  number       = {45},
  pages        = {12892--12906},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Interactions of PAMAM Dendrimers with Negatively Charged Model Biomembranes.},
  url          = {http://dx.doi.org/10.1021/jp506510s},
  volume       = {118},
  year         = {2014},
}