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Acoustic Trapping for Bacteria Identification in Positive Blood Cultures with MALDI-TOF MS.

Hammarström, Björn; Nilson, Bo LU ; Laurell, Thomas LU ; Nilsson, Johan and Ekström, Simon (2014) In Analytical Chemistry 86(21). p.10560-10567
Abstract
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently changing the clinical routine for identification of microbial pathogens. One important application is the rapid identification of bacteria for the diagnosis of bloodstream infections (BSI). A novel approach based on acoustic trapping and an integrated selective enrichment target (ISET) microchip that improves the sample preparation step for this type of analysis is presented. The method is evaluated on clinically relevant samples in the form of Escherichia coli infected blood cultures. It is shown that noncontact acoustic trapping enables capture, enrichment, and washing of bacteria directly from the complex background of crude blood... (More)
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently changing the clinical routine for identification of microbial pathogens. One important application is the rapid identification of bacteria for the diagnosis of bloodstream infections (BSI). A novel approach based on acoustic trapping and an integrated selective enrichment target (ISET) microchip that improves the sample preparation step for this type of analysis is presented. The method is evaluated on clinically relevant samples in the form of Escherichia coli infected blood cultures. It is shown that noncontact acoustic trapping enables capture, enrichment, and washing of bacteria directly from the complex background of crude blood cultures. The technology replaces centrifugation-based separation with a faster and highly automated sample preparation method that minimizes manual handling of hazardous pathogens. The presented method includes a solid phase extraction step that was optimized for enrichment of the bacterial proteins and peptides that are used for bacterial identification. The acoustic trapping-based assay provided correct identification in 12 out 12 cases of E. coli positive blood cultures with an average score of 2.19 ± 0.09 compared to 1.98 ± 0.08 when using the standard assay. This new technology opens up the possibility to automate and speed up an important and widely used diagnostic assay for bloodstream infections. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
86
issue
21
pages
10560 - 10567
publisher
The American Chemical Society
external identifiers
  • pmid:25269087
  • wos:000344510200012
  • scopus:84908568716
ISSN
1520-6882
DOI
10.1021/ac502020f
language
English
LU publication?
yes
id
d4d30fd0-c42b-4765-ace4-3d50ca93f313 (old id 4738723)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25269087?dopt=Abstract
date added to LUP
2014-11-04 18:53:48
date last changed
2017-10-01 03:21:39
@article{d4d30fd0-c42b-4765-ace4-3d50ca93f313,
  abstract     = {Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently changing the clinical routine for identification of microbial pathogens. One important application is the rapid identification of bacteria for the diagnosis of bloodstream infections (BSI). A novel approach based on acoustic trapping and an integrated selective enrichment target (ISET) microchip that improves the sample preparation step for this type of analysis is presented. The method is evaluated on clinically relevant samples in the form of Escherichia coli infected blood cultures. It is shown that noncontact acoustic trapping enables capture, enrichment, and washing of bacteria directly from the complex background of crude blood cultures. The technology replaces centrifugation-based separation with a faster and highly automated sample preparation method that minimizes manual handling of hazardous pathogens. The presented method includes a solid phase extraction step that was optimized for enrichment of the bacterial proteins and peptides that are used for bacterial identification. The acoustic trapping-based assay provided correct identification in 12 out 12 cases of E. coli positive blood cultures with an average score of 2.19 ± 0.09 compared to 1.98 ± 0.08 when using the standard assay. This new technology opens up the possibility to automate and speed up an important and widely used diagnostic assay for bloodstream infections.},
  author       = {Hammarström, Björn and Nilson, Bo and Laurell, Thomas and Nilsson, Johan and Ekström, Simon},
  issn         = {1520-6882},
  language     = {eng},
  number       = {21},
  pages        = {10560--10567},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Acoustic Trapping for Bacteria Identification in Positive Blood Cultures with MALDI-TOF MS.},
  url          = {http://dx.doi.org/10.1021/ac502020f},
  volume       = {86},
  year         = {2014},
}