Dipeptidyl peptidase-4 (DPP-4): Localization and activity in human and rodent islets.
(2014) In Biochemical and Biophysical Research Communications 453(3). p.398-404- Abstract
- Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and... (More)
- Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and inhibition of DPP-4 enhanced insulin secretion in the presence of glucagon-like peptide-1 (GLP-1) in the cells. DPP-4 activity was remarkably robust when cultured with high glucose, incretin hormones, and insulin in mouse and human islets as well as INS-1 832/13 cells and islet DPP-4 activity and expression pattern was not altered in double incretin receptor knockout mice, compared to wild type mice. We conclude that islet DPP-4 is species-specifically expressed in α-cell and β-cell dominant patterns in several species and both patterns remained robust in enzyme activity during short-term metabolic challenge. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4738731
- author
- Liu, Liehua ; Omar, Bilal LU ; Marchetti, Piero and Ahrén, Bo LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 453
- issue
- 3
- pages
- 398 - 404
- publisher
- Elsevier
- external identifiers
-
- pmid:25268763
- wos:000350267500019
- scopus:84918588644
- pmid:25268763
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2014.09.096
- language
- English
- LU publication?
- yes
- id
- f6ea2ebc-dc41-45bb-b6cb-b84c6f9bc07b (old id 4738731)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25268763?dopt=Abstract
- date added to LUP
- 2016-04-01 10:44:07
- date last changed
- 2024-12-02 18:23:39
@article{f6ea2ebc-dc41-45bb-b6cb-b84c6f9bc07b, abstract = {{Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and inhibition of DPP-4 enhanced insulin secretion in the presence of glucagon-like peptide-1 (GLP-1) in the cells. DPP-4 activity was remarkably robust when cultured with high glucose, incretin hormones, and insulin in mouse and human islets as well as INS-1 832/13 cells and islet DPP-4 activity and expression pattern was not altered in double incretin receptor knockout mice, compared to wild type mice. We conclude that islet DPP-4 is species-specifically expressed in α-cell and β-cell dominant patterns in several species and both patterns remained robust in enzyme activity during short-term metabolic challenge.}}, author = {{Liu, Liehua and Omar, Bilal and Marchetti, Piero and Ahrén, Bo}}, issn = {{1090-2104}}, language = {{eng}}, number = {{3}}, pages = {{398--404}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Dipeptidyl peptidase-4 (DPP-4): Localization and activity in human and rodent islets.}}, url = {{http://dx.doi.org/10.1016/j.bbrc.2014.09.096}}, doi = {{10.1016/j.bbrc.2014.09.096}}, volume = {{453}}, year = {{2014}}, }