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Dipeptidyl peptidase-4 (DPP-4): Localization and activity in human and rodent islets.

Liu, Liehua; Omar, Bilal LU ; Marchetti, Piero and Ahrén, Bo LU (2014) In Biochemical and Biophysical Research Communications 453(3). p.398-404
Abstract
Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and... (More)
Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and inhibition of DPP-4 enhanced insulin secretion in the presence of glucagon-like peptide-1 (GLP-1) in the cells. DPP-4 activity was remarkably robust when cultured with high glucose, incretin hormones, and insulin in mouse and human islets as well as INS-1 832/13 cells and islet DPP-4 activity and expression pattern was not altered in double incretin receptor knockout mice, compared to wild type mice. We conclude that islet DPP-4 is species-specifically expressed in α-cell and β-cell dominant patterns in several species and both patterns remained robust in enzyme activity during short-term metabolic challenge. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
453
issue
3
pages
398 - 404
publisher
Elsevier
external identifiers
  • pmid:25268763
  • wos:000350267500019
  • scopus:84918588644
ISSN
1090-2104
DOI
10.1016/j.bbrc.2014.09.096
language
English
LU publication?
yes
id
f6ea2ebc-dc41-45bb-b6cb-b84c6f9bc07b (old id 4738731)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25268763?dopt=Abstract
date added to LUP
2014-11-04 18:55:14
date last changed
2017-09-03 03:23:53
@article{f6ea2ebc-dc41-45bb-b6cb-b84c6f9bc07b,
  abstract     = {Dipeptidyl peptidase 4 (DPP-4) was recently found to be expressed in human and mouse islets with different expression patterns. However, whether species-dependent expression pattern is a generalized phenomenon and whether islet DPP-4 activity is regulated are not known. This study was conducted to investigate DPP-4 localization in several different species, and to examine the impact of glucose, incretin hormones, and insulin on islet DPP-4 activity. It was shown by immuofluorescent staining that there were two distinct species-specific expression patterns of islet DPP-4. The enzyme was expressed exclusively in α-cells in human and pig islets, but primarily in β-cells in mouse and rat islets. INS-1 832/13 cells also expressed DPP-4, and inhibition of DPP-4 enhanced insulin secretion in the presence of glucagon-like peptide-1 (GLP-1) in the cells. DPP-4 activity was remarkably robust when cultured with high glucose, incretin hormones, and insulin in mouse and human islets as well as INS-1 832/13 cells and islet DPP-4 activity and expression pattern was not altered in double incretin receptor knockout mice, compared to wild type mice. We conclude that islet DPP-4 is species-specifically expressed in α-cell and β-cell dominant patterns in several species and both patterns remained robust in enzyme activity during short-term metabolic challenge.},
  author       = {Liu, Liehua and Omar, Bilal and Marchetti, Piero and Ahrén, Bo},
  issn         = {1090-2104},
  language     = {eng},
  number       = {3},
  pages        = {398--404},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Dipeptidyl peptidase-4 (DPP-4): Localization and activity in human and rodent islets.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2014.09.096},
  volume       = {453},
  year         = {2014},
}