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Zinc transporter 8 autoantibodies in patients with type 1 diabetes from a multiethnic population and their first degree relatives.

Araujo, Débora Batista; Skärstrand, Hanna LU ; Barone, Bianca; Dantas, Joana Rodrigues; Kupfer, Rosane; Zajdenverg, Lenita; Milech, Adolpho; Vaziri Sani, Fariba LU ; Oliveira, José Egídio Paulo de and Rodacki, Melanie (2014) In Arquivos Brasileiros de Endocrinologia e Metabologia 58(7). p.737-743
Abstract
Objective Zinc transporter 8 autoantibodies (ZnT8A) have been poorly studied in non-Caucasian individuals. We aimed to investigate the prevalence of ZnT8 autoantibodies in patients with T1D and their first degree relatives (FDR) from a multiethnic population, as well as its relation with the insulin (INS) or the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene polymorphisms. Subjects and methods ZnT8A were analyzed in sera from T1D patients (n = 72, mean age of 30.3 ± 11.4 years) of variable duration (15.7 ± 11.8 years) and their FDR (n = 78, mean age of 18.3 ± 9.1 years) by a triple mix Radioligand Binding Assay (RBA) for the ZnT8 autoantibody (ZnT8-RWQ) variants. SNP (single nucleotide polymorphism) for INS and PTPN22 were... (More)
Objective Zinc transporter 8 autoantibodies (ZnT8A) have been poorly studied in non-Caucasian individuals. We aimed to investigate the prevalence of ZnT8 autoantibodies in patients with T1D and their first degree relatives (FDR) from a multiethnic population, as well as its relation with the insulin (INS) or the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene polymorphisms. Subjects and methods ZnT8A were analyzed in sera from T1D patients (n = 72, mean age of 30.3 ± 11.4 years) of variable duration (15.7 ± 11.8 years) and their FDR (n = 78, mean age of 18.3 ± 9.1 years) by a triple mix Radioligand Binding Assay (RBA) for the ZnT8 autoantibody (ZnT8-RWQ) variants. SNP (single nucleotide polymorphism) for INS and PTPN22 were genotyped. Results The prevalence of ZnT8A was higher in T1D patients than FDR, for ZnT8TripleA (24% vs. 4%,p = 0.001), ZnT8RA (24% vs. 4%, p < 0.001) and ZnT8QA (15% vs. 3%, p = 0.004). All FDR with ZnT8A (n = 3) had at least another positive antibody. Heterozygosis for PTPN22 was associated with a higher frequency of ZnT8TripleA (p = 0.039) and ZnT8RA (p = 0.038). Conclusions ZnT8A is observed in non-Caucasian patients with T1D, even years after the disease onset, as well as in their FDR. In those, there was an overlap between ZnT8A and other T1D antibodies. ZnT8A was associated with PTPN22 polymorphisms. Further longitudinal studies are necessary to elucidate the importance of these findings in the natural history of T1D patients with multiethnic background. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
Arquivos Brasileiros de Endocrinologia e Metabologia
volume
58
issue
7
pages
737 - 743
publisher
SBEM-SOC BRASIL ENDOCRINOLOGIA & METABOLOGIA
external identifiers
  • pmid:25372583
  • wos:000344466000007
  • scopus:84908366874
ISSN
1677-9487
DOI
10.1590/0004-2730000003088
language
English
LU publication?
yes
id
31b03d4d-27f6-4162-9af6-a4ac90361f1e (old id 4820207)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25372583?dopt=Abstract
date added to LUP
2014-12-01 19:14:15
date last changed
2017-04-09 03:24:55
@article{31b03d4d-27f6-4162-9af6-a4ac90361f1e,
  abstract     = {Objective Zinc transporter 8 autoantibodies (ZnT8A) have been poorly studied in non-Caucasian individuals. We aimed to investigate the prevalence of ZnT8 autoantibodies in patients with T1D and their first degree relatives (FDR) from a multiethnic population, as well as its relation with the insulin (INS) or the protein tyrosine phosphatase non-receptor 22 (PTPN22) gene polymorphisms. Subjects and methods ZnT8A were analyzed in sera from T1D patients (n = 72, mean age of 30.3 ± 11.4 years) of variable duration (15.7 ± 11.8 years) and their FDR (n = 78, mean age of 18.3 ± 9.1 years) by a triple mix Radioligand Binding Assay (RBA) for the ZnT8 autoantibody (ZnT8-RWQ) variants. SNP (single nucleotide polymorphism) for INS and PTPN22 were genotyped. Results The prevalence of ZnT8A was higher in T1D patients than FDR, for ZnT8TripleA (24% vs. 4%,p = 0.001), ZnT8RA (24% vs. 4%, p &lt; 0.001) and ZnT8QA (15% vs. 3%, p = 0.004). All FDR with ZnT8A (n = 3) had at least another positive antibody. Heterozygosis for PTPN22 was associated with a higher frequency of ZnT8TripleA (p = 0.039) and ZnT8RA (p = 0.038). Conclusions ZnT8A is observed in non-Caucasian patients with T1D, even years after the disease onset, as well as in their FDR. In those, there was an overlap between ZnT8A and other T1D antibodies. ZnT8A was associated with PTPN22 polymorphisms. Further longitudinal studies are necessary to elucidate the importance of these findings in the natural history of T1D patients with multiethnic background.},
  author       = {Araujo, Débora Batista and Skärstrand, Hanna and Barone, Bianca and Dantas, Joana Rodrigues and Kupfer, Rosane and Zajdenverg, Lenita and Milech, Adolpho and Vaziri Sani, Fariba and Oliveira, José Egídio Paulo de and Rodacki, Melanie},
  issn         = {1677-9487},
  language     = {eng},
  number       = {7},
  pages        = {737--743},
  publisher    = {SBEM-SOC BRASIL ENDOCRINOLOGIA & METABOLOGIA},
  series       = {Arquivos Brasileiros de Endocrinologia e Metabologia},
  title        = {Zinc transporter 8 autoantibodies in patients with type 1 diabetes from a multiethnic population and their first degree relatives.},
  url          = {http://dx.doi.org/10.1590/0004-2730000003088},
  volume       = {58},
  year         = {2014},
}