SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION
(2022) 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.306-307- Abstract
Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/484f1a63-6814-42d3-b614-de3ca9b0a240
- author
- Akbari, Elham LU ; Beech, Jason P. LU ; Wrighton, Sebastian LU ; Nordenfelt, Pontus LU and Tegenfeldt, Jonas O. LU
- organization
- publishing date
- 2022
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- bacteria clusters, Deterministic Lateral Displacement, filter-sonication, group A streptococci, label free separation
- host publication
- MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- series title
- MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
- conference location
- Hybrid, Hangzhou, China
- conference dates
- 2022-10-23 - 2022-10-27
- external identifiers
-
- scopus:85175722093
- ISBN
- 9781733419048
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
- id
- 484f1a63-6814-42d3-b614-de3ca9b0a240
- date added to LUP
- 2023-11-23 21:36:38
- date last changed
- 2024-03-26 11:43:16
@inproceedings{484f1a63-6814-42d3-b614-de3ca9b0a240, abstract = {{<p>Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.</p>}}, author = {{Akbari, Elham and Beech, Jason P. and Wrighton, Sebastian and Nordenfelt, Pontus and Tegenfeldt, Jonas O.}}, booktitle = {{MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419048}}, keywords = {{bacteria clusters; Deterministic Lateral Displacement; filter-sonication; group A streptococci; label free separation}}, language = {{eng}}, pages = {{306--307}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, title = {{SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION}}, year = {{2022}}, }