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SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION

Akbari, Elham LU ; Beech, Jason P. LU ; Wrighton, Sebastian LU orcid ; Nordenfelt, Pontus LU orcid and Tegenfeldt, Jonas O. LU orcid (2022) 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 In MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.306-307
Abstract

Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.

Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
bacteria clusters, Deterministic Lateral Displacement, filter-sonication, group A streptococci, label free separation
host publication
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
series title
MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
conference location
Hybrid, Hangzhou, China
conference dates
2022-10-23 - 2022-10-27
external identifiers
  • scopus:85175722093
ISBN
9781733419048
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
id
484f1a63-6814-42d3-b614-de3ca9b0a240
date added to LUP
2023-11-23 21:36:38
date last changed
2024-03-26 11:43:16
@inproceedings{484f1a63-6814-42d3-b614-de3ca9b0a240,
  abstract     = {{<p>Differences in morphologies of bacteria and bacteria clusters are thought to contribute to their virulence and colonization. However, the conventional standard cell biological methods cannot separate bacteria and bacteria clusters based on their morphologies and sizes, making studies of the underlying mechanisms difficult. Here we report a simple label-free method for the continuous separation of singlets and clusters, of group A streptococci, based on their size and morphology, using Deterministic Lateral Displacement and filter-sonication. In general, this opens up for the generation of cell populations with heterogenicity in cluster size and physical properties.</p>}},
  author       = {{Akbari, Elham and Beech, Jason P. and Wrighton, Sebastian and Nordenfelt, Pontus and Tegenfeldt, Jonas O.}},
  booktitle    = {{MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419048}},
  keywords     = {{bacteria clusters; Deterministic Lateral Displacement; filter-sonication; group A streptococci; label free separation}},
  language     = {{eng}},
  pages        = {{306--307}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  title        = {{SEPARATION OF SINGLETS AND CLUSTERS OF GROUP A STREPTOCOCCI USING DETERMINISTIC LATERAL DISPLACEMENT AND FILTER SONICATION}},
  year         = {{2022}},
}