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Acoustofluidics 8: Applications of acoustophoresis in continuous flow microsystems

Lenshof, Andreas LU ; Magnusson, Cecilia LU and Laurell, Thomas LU (2012) In Lab on A Chip 12(7). p.1210-1223
Abstract
This acoustofluidics tutorial focuses on continuous flow-based half wavelength resonator systems operated in the transversal mode, where the direction of the primary acoustic force acts in plane with the microchip. The transversal actuation mode facilitates integration with up- and downstream microchannel networks as well as visual control of the acoustic focusing experiment. Applications of particle enrichment in an acoustic half wavelength resonator are discussed as well as clarification of the carrier fluid from undesired particles. Binary separation of particle/vesicle/cell mixtures into two subpopulations is outlined based on the different polarities of the acoustic contrast factor. Furthermore, continuous flow separation of different... (More)
This acoustofluidics tutorial focuses on continuous flow-based half wavelength resonator systems operated in the transversal mode, where the direction of the primary acoustic force acts in plane with the microchip. The transversal actuation mode facilitates integration with up- and downstream microchannel networks as well as visual control of the acoustic focusing experiment. Applications of particle enrichment in an acoustic half wavelength resonator are discussed as well as clarification of the carrier fluid from undesired particles. Binary separation of particle/vesicle/cell mixtures into two subpopulations is outlined based on the different polarities of the acoustic contrast factor. Furthermore, continuous flow separation of different particle/cell types is described where both Free Flow Acoustophoresis (FFA) and binary acoustophoresis are utilized. By capitalizing on the laminar flow regime, acoustophoresis has proven especially successful in performing bead/cell translations between different buffer systems. Likewise, the ability to controllably translate particulate matter across streamlines has opened a route to valving of cells/particles without any moving parts, where event triggered cell sorting is becoming an increasing area of activity. Recent developments now also enable measurements of fundamental cell properties such as density and compressibility by means of acoustophoresis. General aspects on working with live cells in acoustophoresis systems are discussed as well as available means to quantify the outcome of cell and particle separation experiments performed by acoustophoresis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
12
issue
7
pages
1210 - 1223
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84857989931
  • wos:000301196700001
  • pmid:22362021
ISSN
1473-0189
DOI
10.1039/C2LC21256Khttp://dx.doi.org/10.1039/c2lc21256k
language
English
LU publication?
yes
id
a84410b7-789c-48c6-a43f-77950c023cb9 (old id 4857892)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22362021?dopt=Abstract
date added to LUP
2014-12-08 15:58:22
date last changed
2017-09-03 03:32:06
@article{a84410b7-789c-48c6-a43f-77950c023cb9,
  abstract     = {This acoustofluidics tutorial focuses on continuous flow-based half wavelength resonator systems operated in the transversal mode, where the direction of the primary acoustic force acts in plane with the microchip. The transversal actuation mode facilitates integration with up- and downstream microchannel networks as well as visual control of the acoustic focusing experiment. Applications of particle enrichment in an acoustic half wavelength resonator are discussed as well as clarification of the carrier fluid from undesired particles. Binary separation of particle/vesicle/cell mixtures into two subpopulations is outlined based on the different polarities of the acoustic contrast factor. Furthermore, continuous flow separation of different particle/cell types is described where both Free Flow Acoustophoresis (FFA) and binary acoustophoresis are utilized. By capitalizing on the laminar flow regime, acoustophoresis has proven especially successful in performing bead/cell translations between different buffer systems. Likewise, the ability to controllably translate particulate matter across streamlines has opened a route to valving of cells/particles without any moving parts, where event triggered cell sorting is becoming an increasing area of activity. Recent developments now also enable measurements of fundamental cell properties such as density and compressibility by means of acoustophoresis. General aspects on working with live cells in acoustophoresis systems are discussed as well as available means to quantify the outcome of cell and particle separation experiments performed by acoustophoresis.},
  author       = {Lenshof, Andreas and Magnusson, Cecilia and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {7},
  pages        = {1210--1223},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Acoustofluidics 8: Applications of acoustophoresis in continuous flow microsystems},
  url          = {http://dx.doi.org/10.1039/C2LC21256Khttp://dx.doi.org/10.1039/c2lc21256k},
  volume       = {12},
  year         = {2012},
}