Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements.
(2010) In Cytotechnology 62. p.167-174- Abstract
- Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to... (More)
- Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1595109
- author
- Schartau, Marcus LU ; Kröger, Ronald LU and Sjögreen, Bodil LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Optical measurements, Teleost, Lens, Organ culture
- in
- Cytotechnology
- volume
- 62
- pages
- 167 - 174
- publisher
- Springer
- external identifiers
-
- wos:000279053700009
- scopus:77955717398
- pmid:20401633
- ISSN
- 0920-9069
- DOI
- 10.1007/s10616-010-9268-y
- language
- English
- LU publication?
- yes
- id
- 487061bf-a41b-4118-8679-407aff01c570 (old id 1595109)
- date added to LUP
- 2016-04-01 14:54:29
- date last changed
- 2022-01-28 03:04:35
@article{487061bf-a41b-4118-8679-407aff01c570,
abstract = {{Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment.}},
author = {{Schartau, Marcus and Kröger, Ronald and Sjögreen, Bodil}},
issn = {{0920-9069}},
keywords = {{Optical measurements; Teleost; Lens; Organ culture}},
language = {{eng}},
pages = {{167--174}},
publisher = {{Springer}},
series = {{Cytotechnology}},
title = {{Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements.}},
url = {{http://dx.doi.org/10.1007/s10616-010-9268-y}},
doi = {{10.1007/s10616-010-9268-y}},
volume = {{62}},
year = {{2010}},
}