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Age-dependent effect of obestatin on intestinal contractility in Wistar rats

Slupecka, M.; Pierzynowski, Stefan LU ; Kuwahara, A.; Kato, I. and Wolinski, J. (2014) In General and Comparative Endocrinology 208. p.109-115
Abstract
Obestatin is a 23-amino acid peptide encoded by the ghrelin gene. We have investigated the effect of obestatin on intestinal contractility in rats ranging from the suckling period till adolescence. Duodenal and middle jejunum whole-thickness preparations from neonatal and adult rats were studied in an organ bath, for isometric recording under treatment with obestatin (1 mu mol L-1) in the presence of acetylocholine (ACh), atropine and tetradotoxin (TTX). Both the EFS and ACh-stimulated contractile response, as well as spontaneous contractile activity is age-dependent and specific for the segment of jejunum. Except for the middle jejunum of 7 day old rats, treatment with obestatin caused a significant TTX-sensitive increase in the amplitude... (More)
Obestatin is a 23-amino acid peptide encoded by the ghrelin gene. We have investigated the effect of obestatin on intestinal contractility in rats ranging from the suckling period till adolescence. Duodenal and middle jejunum whole-thickness preparations from neonatal and adult rats were studied in an organ bath, for isometric recording under treatment with obestatin (1 mu mol L-1) in the presence of acetylocholine (ACh), atropine and tetradotoxin (TTX). Both the EFS and ACh-stimulated contractile response, as well as spontaneous contractile activity is age-dependent and specific for the segment of jejunum. Except for the middle jejunum of 7 day old rats, treatment with obestatin caused a significant TTX-sensitive increase in the amplitude of EFS-stimulated off-contraction of both intestinal segments studied. Following injection of obestatin, the amplitude of spontaneous contraction in the duodenum increased in 7 day old rats. In the middle jejunum, treatment with obestatin significantly increased both the amplitude and frequency of spontaneous contraction in rats till the 28th day of life, whereas in adult rats the observed effect of obestatin was the opposite (P < 0.001 and P < 0.0001, respectively). The effects of treatment with obestatin on stimulation with increasing doses of ACh were only observed in the preparations from suckling rats. ACh-stimulated contractility in the duodenum was decreased while in the middle jejunum the observed effect was opposite. These results indicate the importance of peripheral obestatin in the cholinergic control of intestinal contractility in both neonatal and adult rats. (C) 2014 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Obestatin, Intestine, Contractility, Neonates
in
General and Comparative Endocrinology
volume
208
pages
109 - 115
publisher
Elsevier
external identifiers
  • wos:000344209000013
  • pmid:25193331
  • scopus:84907499762
ISSN
0016-6480
DOI
10.1016/j.ygcen.2014.08.015
language
English
LU publication?
yes
id
df780d9d-9c54-472b-9ee8-8bf39f342e53 (old id 4875069)
date added to LUP
2015-01-07 10:25:22
date last changed
2017-06-11 03:01:22
@article{df780d9d-9c54-472b-9ee8-8bf39f342e53,
  abstract     = {Obestatin is a 23-amino acid peptide encoded by the ghrelin gene. We have investigated the effect of obestatin on intestinal contractility in rats ranging from the suckling period till adolescence. Duodenal and middle jejunum whole-thickness preparations from neonatal and adult rats were studied in an organ bath, for isometric recording under treatment with obestatin (1 mu mol L-1) in the presence of acetylocholine (ACh), atropine and tetradotoxin (TTX). Both the EFS and ACh-stimulated contractile response, as well as spontaneous contractile activity is age-dependent and specific for the segment of jejunum. Except for the middle jejunum of 7 day old rats, treatment with obestatin caused a significant TTX-sensitive increase in the amplitude of EFS-stimulated off-contraction of both intestinal segments studied. Following injection of obestatin, the amplitude of spontaneous contraction in the duodenum increased in 7 day old rats. In the middle jejunum, treatment with obestatin significantly increased both the amplitude and frequency of spontaneous contraction in rats till the 28th day of life, whereas in adult rats the observed effect of obestatin was the opposite (P &lt; 0.001 and P &lt; 0.0001, respectively). The effects of treatment with obestatin on stimulation with increasing doses of ACh were only observed in the preparations from suckling rats. ACh-stimulated contractility in the duodenum was decreased while in the middle jejunum the observed effect was opposite. These results indicate the importance of peripheral obestatin in the cholinergic control of intestinal contractility in both neonatal and adult rats. (C) 2014 Elsevier Inc. All rights reserved.},
  author       = {Slupecka, M. and Pierzynowski, Stefan and Kuwahara, A. and Kato, I. and Wolinski, J.},
  issn         = {0016-6480},
  keyword      = {Obestatin,Intestine,Contractility,Neonates},
  language     = {eng},
  pages        = {109--115},
  publisher    = {Elsevier},
  series       = {General and Comparative Endocrinology},
  title        = {Age-dependent effect of obestatin on intestinal contractility in Wistar rats},
  url          = {http://dx.doi.org/10.1016/j.ygcen.2014.08.015},
  volume       = {208},
  year         = {2014},
}