Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat
(1991) In Pflügers Archiv 419(3-4). p.230-234- Abstract
- The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal... (More)
- The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal vein and urinary bladder. In the hypertrophic urinary bladder a shift in LDH isoform pattern towards less of the H form was found, which correlates with a better maintenance of contraction in anoxia in this type of hypertrophic smooth muscle. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106034
- author
- Malmqvist, Ulf LU ; Arner, Anders LU and Uvelius, Bengt LU
- organization
- publishing date
- 1991
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lactate dehydrohgenase, Smooth muscle, Isoforms, Hypertrophy, Rat urinary bladder, Rat portal vein, Rat arota
- in
- Pflügers Archiv
- volume
- 419
- issue
- 3-4
- pages
- 230 - 234
- publisher
- Springer
- external identifiers
-
- pmid:1745598
- scopus:0026012306
- ISSN
- 0031-6768
- DOI
- 10.1007/BF00371100
- language
- English
- LU publication?
- yes
- id
- 488a955f-75c3-4860-8489-d9200f83bb96 (old id 1106034)
- date added to LUP
- 2016-04-01 16:24:03
- date last changed
- 2021-12-23 08:14:45
@article{488a955f-75c3-4860-8489-d9200f83bb96, abstract = {{The lactate dehydrogenase (LDH) activity and isoform distribution of LDH were investigated in tissue samples from the rat portal vein, aorta and urinary bladder. In addition, samples were obtained from hypertrophic urinary bladder. The total LDH activity per unit smooth muscle volume was higher in the urinary bladder compared to that in portal vein and aorta. Five LDH isoforms, reflecting different combinations of the two polypeptide chains denoted H and M, could be separated by agarose gel electrophoresis. The aorta contained more of the H form compared to the portal vein and urinary bladder. This difference suggests that the aorta, which is a slow smooth muscle, is more adapted for aerobic metabolism than the faster muscles of portal vein and urinary bladder. In the hypertrophic urinary bladder a shift in LDH isoform pattern towards less of the H form was found, which correlates with a better maintenance of contraction in anoxia in this type of hypertrophic smooth muscle.}}, author = {{Malmqvist, Ulf and Arner, Anders and Uvelius, Bengt}}, issn = {{0031-6768}}, keywords = {{Lactate dehydrohgenase; Smooth muscle; Isoforms; Hypertrophy; Rat urinary bladder; Rat portal vein; Rat arota}}, language = {{eng}}, number = {{3-4}}, pages = {{230--234}}, publisher = {{Springer}}, series = {{Pflügers Archiv}}, title = {{Lactate dehydrogenase activity and isoform distribution in normal and hypertrophic smooth muscle tissue from the rat}}, url = {{http://dx.doi.org/10.1007/BF00371100}}, doi = {{10.1007/BF00371100}}, volume = {{419}}, year = {{1991}}, }