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Semicarbazide-functionalized resin as a new scavenger for in situ recovery of 3-hydroxypropionaldehyde during biotransformation of glycerol by Lactobacillus reuteri

Rezaei, Roya LU ; Dishisha, Tarek LU ; Pyo, Sang-Hyun LU and Hatti-Kaul, Rajni LU (2014) In Journal of Biotechnology 192(A). p.223-230
Abstract
3-Hydroxypropionaldehyde (3HPA), a potential C3-platform chemical for a biobased industry, is produced from glycerol using Lactobacillus reuteri through its glycerol dehydratase activity. However, the process is characterized by low yield and productivity due to toxic effects of 3HPA on the biocatalyst activity. In this study, a semicarbazide-functionalized resin was prepared, evaluated for adsorption and in situ recovery of 3HPA during biotransformation of glycerol. Adsorption of 3HPA onto the resin was characterized as “S-curve model”, increasing with increasing initial 3HPA concentration, and reached a maximum of 9.48 mmol/gresin at 71.54 mM 3HPA used. Desorption of 3HPA was evaluated using water and different acids, and was enhanced by... (More)
3-Hydroxypropionaldehyde (3HPA), a potential C3-platform chemical for a biobased industry, is produced from glycerol using Lactobacillus reuteri through its glycerol dehydratase activity. However, the process is characterized by low yield and productivity due to toxic effects of 3HPA on the biocatalyst activity. In this study, a semicarbazide-functionalized resin was prepared, evaluated for adsorption and in situ recovery of 3HPA during biotransformation of glycerol. Adsorption of 3HPA onto the resin was characterized as “S-curve model”, increasing with increasing initial 3HPA concentration, and reached a maximum of 9.48 mmol/gresin at 71.54 mM 3HPA used. Desorption of 3HPA was evaluated using water and different acids, and was enhanced by acetic acid with organic modifiers. Repeated adsorption–desorption of 3HPA in batch resulted in elution of 13–66.5% of the bound 3HPA during at least three sequential cycles using water and acetic acid, respectively as eluants. Using the resin for in situ product removal led to more than 2 times higher productivity of 3HPA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3-Hydroxypropionaldehyde, Lactobacillus reuteri, Semicarbazide-functionalized resin, Whole cell biotransformation, In situ product removal
in
Journal of Biotechnology
volume
192
issue
A
pages
223 - 230
publisher
Elsevier
external identifiers
  • pmid:25456063
  • wos:000345970300036
  • scopus:84909975258
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2014.10.013
language
English
LU publication?
yes
id
096e65c8-ffdc-4c0e-a29b-b16e150b1924 (old id 4897183)
date added to LUP
2015-01-12 13:33:11
date last changed
2017-03-05 03:04:08
@article{096e65c8-ffdc-4c0e-a29b-b16e150b1924,
  abstract     = {3-Hydroxypropionaldehyde (3HPA), a potential C3-platform chemical for a biobased industry, is produced from glycerol using Lactobacillus reuteri through its glycerol dehydratase activity. However, the process is characterized by low yield and productivity due to toxic effects of 3HPA on the biocatalyst activity. In this study, a semicarbazide-functionalized resin was prepared, evaluated for adsorption and in situ recovery of 3HPA during biotransformation of glycerol. Adsorption of 3HPA onto the resin was characterized as “S-curve model”, increasing with increasing initial 3HPA concentration, and reached a maximum of 9.48 mmol/gresin at 71.54 mM 3HPA used. Desorption of 3HPA was evaluated using water and different acids, and was enhanced by acetic acid with organic modifiers. Repeated adsorption–desorption of 3HPA in batch resulted in elution of 13–66.5% of the bound 3HPA during at least three sequential cycles using water and acetic acid, respectively as eluants. Using the resin for in situ product removal led to more than 2 times higher productivity of 3HPA.},
  author       = {Rezaei, Roya and Dishisha, Tarek and Pyo, Sang-Hyun and Hatti-Kaul, Rajni},
  issn         = {1873-4863},
  keyword      = {3-Hydroxypropionaldehyde,Lactobacillus reuteri,Semicarbazide-functionalized resin,Whole cell biotransformation,In situ product removal},
  language     = {eng},
  number       = {A},
  pages        = {223--230},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Semicarbazide-functionalized resin as a new scavenger for in situ recovery of 3-hydroxypropionaldehyde during biotransformation of glycerol by Lactobacillus reuteri},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2014.10.013},
  volume       = {192},
  year         = {2014},
}