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The clinicopathological and gene expression patterns associated with ulceration of primary melanoma

Jewell, Rosalyn; Elliott, Faye; Laye, Jonathan; Nsengimana, Jeremie; Davies, John; Walker, Christy; Conway, Caroline; Mitra, Angana; Harland, Mark and Cook, Martin G., et al. (2015) In Pigment Cell & Melanoma Research 28(1).
Abstract
Ulceration of primary melanomas is associated with poor prognosis yet is reported to predict benefit from adjuvant interferon. To better understand the biological processes involved, clinicopathological factors associated with ulceration were determined in 1804 patients. From this cohort, 348 primary tumor blocks were sampled to generate gene expression data using a 502-gene cancer panel and 195 blocks were used for immunohistochemistry to detect macrophage infiltration and vessel density. Gene expression results were validated using a whole genome array in two independent sample sets. Ulceration of primary melanomas was associated with more proliferative tumors, tumor vessel invasion, and increased microvessel density. Infiltration of... (More)
Ulceration of primary melanomas is associated with poor prognosis yet is reported to predict benefit from adjuvant interferon. To better understand the biological processes involved, clinicopathological factors associated with ulceration were determined in 1804 patients. From this cohort, 348 primary tumor blocks were sampled to generate gene expression data using a 502-gene cancer panel and 195 blocks were used for immunohistochemistry to detect macrophage infiltration and vessel density. Gene expression results were validated using a whole genome array in two independent sample sets. Ulceration of primary melanomas was associated with more proliferative tumors, tumor vessel invasion, and increased microvessel density. Infiltration of tumors with greater number of macrophages and gene expression pathways associated with wound healing and up-regulation of pro-inflammatory cytokines suggests that ulceration is associated with tumor-related inflammation. The relative benefit from interferon reported in patients with ulcerated tumors may reflect modification of signaling pathways involved in inflammation. (Less)
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publication status
published
subject
keywords
melanoma, primary, ulceration, gene expression, clinicopathological, immunohistochemistry
in
Pigment Cell & Melanoma Research
volume
28
issue
1
publisher
Wiley-Blackwell
external identifiers
  • wos:000346826900012
  • scopus:84919846089
ISSN
1755-148X
DOI
10.1111/pcmr.12315
language
English
LU publication?
yes
id
abbeb4c9-2f17-4dfe-a277-f5d8b3f422fe (old id 4941341)
date added to LUP
2015-02-03 07:16:43
date last changed
2017-01-01 04:01:02
@article{abbeb4c9-2f17-4dfe-a277-f5d8b3f422fe,
  abstract     = {Ulceration of primary melanomas is associated with poor prognosis yet is reported to predict benefit from adjuvant interferon. To better understand the biological processes involved, clinicopathological factors associated with ulceration were determined in 1804 patients. From this cohort, 348 primary tumor blocks were sampled to generate gene expression data using a 502-gene cancer panel and 195 blocks were used for immunohistochemistry to detect macrophage infiltration and vessel density. Gene expression results were validated using a whole genome array in two independent sample sets. Ulceration of primary melanomas was associated with more proliferative tumors, tumor vessel invasion, and increased microvessel density. Infiltration of tumors with greater number of macrophages and gene expression pathways associated with wound healing and up-regulation of pro-inflammatory cytokines suggests that ulceration is associated with tumor-related inflammation. The relative benefit from interferon reported in patients with ulcerated tumors may reflect modification of signaling pathways involved in inflammation.},
  author       = {Jewell, Rosalyn and Elliott, Faye and Laye, Jonathan and Nsengimana, Jeremie and Davies, John and Walker, Christy and Conway, Caroline and Mitra, Angana and Harland, Mark and Cook, Martin G. and Boon, Andy and Storr, Sarah and Safuan, Sabreena and Martin, Stewart G. and Jirström, Karin and Olsson, Håkan and Ingvar, Christian and Lauss, Martin and Bishop, Tim and Jönsson, Göran B and Newton-Bishop, Julia},
  issn         = {1755-148X},
  keyword      = {melanoma,primary,ulceration,gene expression,clinicopathological,immunohistochemistry},
  language     = {eng},
  number       = {1},
  publisher    = {Wiley-Blackwell},
  series       = {Pigment Cell & Melanoma Research},
  title        = {The clinicopathological and gene expression patterns associated with ulceration of primary melanoma},
  url          = {http://dx.doi.org/10.1111/pcmr.12315},
  volume       = {28},
  year         = {2015},
}