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Comparative functional analysis of the Caenorhabditis elegans and Drosophila melanogaster proteomes

Schrimpf, Sabine P; Weiss, Manuel; Reiter, Lukas; Ahrens, Christian H.; Jovanovic, Marko; Malmström, Johan LU ; Brunner, Erich; Mohanty, Sonali; Lercher, Martin J and Hunziker, Peter E, et al. (2009) In PLoS Biology 7(3). p.48-48
Abstract

The nematode Caenorhabditis elegans is a popular model system in genetics, not least because a majority of human disease genes are conserved in C. elegans. To generate a comprehensive inventory of its expressed proteome, we performed extensive shotgun proteomics and identified more than half of all predicted C. elegans proteins. This allowed us to confirm and extend genome annotations, characterize the role of operons in C. elegans, and semiquantitatively infer abundance levels for thousands of proteins. Furthermore, for the first time to our knowledge, we were able to compare two animal proteomes (C. elegans and Drosophila melanogaster). We found that the abundances of orthologous proteins in metazoans correlate remarkably well, better... (More)

The nematode Caenorhabditis elegans is a popular model system in genetics, not least because a majority of human disease genes are conserved in C. elegans. To generate a comprehensive inventory of its expressed proteome, we performed extensive shotgun proteomics and identified more than half of all predicted C. elegans proteins. This allowed us to confirm and extend genome annotations, characterize the role of operons in C. elegans, and semiquantitatively infer abundance levels for thousands of proteins. Furthermore, for the first time to our knowledge, we were able to compare two animal proteomes (C. elegans and Drosophila melanogaster). We found that the abundances of orthologous proteins in metazoans correlate remarkably well, better than protein abundance versus transcript abundance within each organism or transcript abundances across organisms; this suggests that changes in transcript abundance may have been partially offset during evolution by opposing changes in protein abundance.

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keywords
Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Drosophila Proteins, Drosophila melanogaster, Gene Duplication, Genome, Operon, Proteome, Proteomics, Sequence Homology, Amino Acid, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
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PLoS Biology
volume
7
issue
3
pages
48 - 48
publisher
Public Library of Science
external identifiers
  • scopus:65949096636
ISSN
1544-9173
DOI
10.1371/journal.pbio.1000048
language
English
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no
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4958e4a3-b3d3-4ecc-9037-d7e896c4a40f
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2017-09-04 17:21:12
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2017-10-04 14:00:17
@article{4958e4a3-b3d3-4ecc-9037-d7e896c4a40f,
  abstract     = {<p>The nematode Caenorhabditis elegans is a popular model system in genetics, not least because a majority of human disease genes are conserved in C. elegans. To generate a comprehensive inventory of its expressed proteome, we performed extensive shotgun proteomics and identified more than half of all predicted C. elegans proteins. This allowed us to confirm and extend genome annotations, characterize the role of operons in C. elegans, and semiquantitatively infer abundance levels for thousands of proteins. Furthermore, for the first time to our knowledge, we were able to compare two animal proteomes (C. elegans and Drosophila melanogaster). We found that the abundances of orthologous proteins in metazoans correlate remarkably well, better than protein abundance versus transcript abundance within each organism or transcript abundances across organisms; this suggests that changes in transcript abundance may have been partially offset during evolution by opposing changes in protein abundance.</p>},
  author       = {Schrimpf, Sabine P and Weiss, Manuel and Reiter, Lukas and Ahrens, Christian H. and Jovanovic, Marko and Malmström, Johan and Brunner, Erich and Mohanty, Sonali and Lercher, Martin J and Hunziker, Peter E and Aebersold, Ruedi and von Mering, Christian and Hengartner, Michael O},
  issn         = {1544-9173},
  keyword      = {Animals,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Drosophila Proteins,Drosophila melanogaster,Gene Duplication,Genome,Operon,Proteome,Proteomics,Sequence Homology, Amino Acid,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {03},
  number       = {3},
  pages        = {48--48},
  publisher    = {Public Library of Science},
  series       = {PLoS Biology},
  title        = {Comparative functional analysis of the Caenorhabditis elegans and Drosophila melanogaster proteomes},
  url          = {http://dx.doi.org/10.1371/journal.pbio.1000048},
  volume       = {7},
  year         = {2009},
}