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S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release

Pichet, Guilherme F. ; Da Silva, Laura C.E. ; Giglio, Leonardo P. ; Plivelic, Tomás LU and de Oliveira, Marcelo G. (2020) In Journal of Colloid and Interface Science 576. p.457-467
Abstract
Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.
Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent... (More)
Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.
Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV–Vis spectrophotometry were used to evaluate the kinetics of NO release.
Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pluronic F127, Hydrogel, Reactive micelles, Microstructure, Nitric oxide, S-nitrosothiol, SAXS, Cryo TEM
in
Journal of Colloid and Interface Science
volume
576
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:85085235617
  • pmid:32470853
ISSN
0021-9797
DOI
10.1016/j.jcis.2020.05.049
language
English
LU publication?
yes
id
49d4d850-952c-4bac-b223-bed2ef71ccdc
date added to LUP
2020-06-06 08:33:33
date last changed
2020-12-29 02:16:20
@article{49d4d850-952c-4bac-b223-bed2ef71ccdc,
  abstract     = {Hypothesis: Nitric oxide (NO)-releasing Pluronic F127 hydrogels (F127) containing dissolved S-nitrosothiols or pendant N-diazeniumdiolate (NONOate) groups have been described. The NO charging of these hydrogels is usually limited by their low stability or disruption of the micellar packing. S-nitrosothiol-terminated F127 may emerge as a new strategy for allowing NO delivery at different rates in biomedical applications.<br/>Experiments: Terminal hydroxyl groups of F127 were esterified and reduced to produce F127-mercaptopropionate (HS-F127-SH), which was subsequently S-nitrosated to generate S-nitrosothiolterminated F127 (ONS-F127-SNO). Micro-differential scanning calorimetry, 1H NMR spin-spin relaxation (T2), temperature-dependent small-angle X-ray scattering, and cryo-transmission electron microscopy, were used to determine the micellar packing structure, while real-time chemiluminescence NO detection and UV–Vis spectrophotometry were used to evaluate the kinetics of NO release.<br/>Findings: HS-F127-SH micellization and gelation processes were analogous to native F127, however, with a decreased short-range ordering of the micelles. ONS-F127-SNO hydrogels released NO thorough a preferentially intramicellar SNO dimerization reaction. Increasing ONS-F127-SNO concentration reduces the rate of SNO dimerization and increases the overall rate of NO release to the gas phase, opening up new possibilities for tailoring NO delivery from F127-based hydrogels.},
  author       = {Pichet, Guilherme F. and Da Silva, Laura C.E. and Giglio, Leonardo P. and Plivelic, Tomás and de Oliveira, Marcelo G.},
  issn         = {0021-9797},
  language     = {eng},
  month        = {05},
  pages        = {457--467},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release},
  url          = {http://dx.doi.org/10.1016/j.jcis.2020.05.049},
  doi          = {10.1016/j.jcis.2020.05.049},
  volume       = {576},
  year         = {2020},
}