Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells
(2023) In Cells 12(10).- Abstract
Bronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little is known regarding the implication of intraepithelial MCs on the local environment, such as epithelial cell function and properties. In this study, we investigated whether MC tryptase is involved in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation. Using novel holographic live cell imaging, we found that MC tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell... (More)
Bronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little is known regarding the implication of intraepithelial MCs on the local environment, such as epithelial cell function and properties. In this study, we investigated whether MC tryptase is involved in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation. Using novel holographic live cell imaging, we found that MC tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell division intervals. The elevated cell growth induced by tryptase remained in a pro-inflammatory state. Tryptase also increased the expression of the anti-apoptotic protein BIRC3, as well as growth factor release in epithelial cells. Thus, our data imply that the intraepithelial and alveolar MC release of tryptase may play a critical role in disturbing bronchial epithelial and alveolar homeostasis by altering cell growth–death regulation.
(Less)
- author
- Berlin, Frida LU ; Mogren, Sofia LU ; Ly, Camilla ; Ramu, Sangeetha LU ; Hvidtfeldt, Morten ; Uller, Lena LU ; Porsbjerg, Celeste and Andersson, Cecilia K. LU
- organization
- publishing date
- 2023-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- airway remodeling, alveolar epithelium, anti-apoptosis, bronchial epithelium, cell growth, growth factors, mast cell, PAR-2, proteases, tryptase
- in
- Cells
- volume
- 12
- issue
- 10
- article number
- 1439
- publisher
- MDPI AG
- external identifiers
-
- pmid:37408273
- scopus:85160634939
- ISSN
- 2073-4409
- DOI
- 10.3390/cells12101439
- language
- English
- LU publication?
- yes
- id
- 4a4d83a6-5974-4f8f-9ac7-8e49bba2c547
- date added to LUP
- 2023-08-23 13:54:34
- date last changed
- 2024-11-02 21:04:42
@article{4a4d83a6-5974-4f8f-9ac7-8e49bba2c547,
abstract = {{<p>Bronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little is known regarding the implication of intraepithelial MCs on the local environment, such as epithelial cell function and properties. In this study, we investigated whether MC tryptase is involved in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation. Using novel holographic live cell imaging, we found that MC tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell division intervals. The elevated cell growth induced by tryptase remained in a pro-inflammatory state. Tryptase also increased the expression of the anti-apoptotic protein BIRC3, as well as growth factor release in epithelial cells. Thus, our data imply that the intraepithelial and alveolar MC release of tryptase may play a critical role in disturbing bronchial epithelial and alveolar homeostasis by altering cell growth–death regulation.</p>}},
author = {{Berlin, Frida and Mogren, Sofia and Ly, Camilla and Ramu, Sangeetha and Hvidtfeldt, Morten and Uller, Lena and Porsbjerg, Celeste and Andersson, Cecilia K.}},
issn = {{2073-4409}},
keywords = {{airway remodeling; alveolar epithelium; anti-apoptosis; bronchial epithelium; cell growth; growth factors; mast cell; PAR-2; proteases; tryptase}},
language = {{eng}},
number = {{10}},
publisher = {{MDPI AG}},
series = {{Cells}},
title = {{Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells}},
url = {{http://dx.doi.org/10.3390/cells12101439}},
doi = {{10.3390/cells12101439}},
volume = {{12}},
year = {{2023}},
}