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A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography

Ghosh, Swagatha ; Zorić, Doris ; Dahl, Peter ; Bjelčić, Monika LU ; Johannesson, Jonatan ; Sandelin, Emil ; Borjesson, Per ; Björling, Alexander LU ; Banacore, Analia and Edlund, Petra , et al. (2023) In Journal of Applied Crystallography 56(Pt 2). p.449-460
Abstract

Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at X-ray free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial crystallography allows the study of structural changes that occur during an enzyme catalytic reaction. Serial synchrotron X-ray crystallography (SSX), which extends serial crystallography methods to synchrotron radiation sources, is expanding the scientific community using serial diffraction methods. This report presents a simple flow cell that can be used to deliver microcrystals across an X-ray beam during SSX studies. This device consists of an X-ray transparent glass capillary... (More)

Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at X-ray free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial crystallography allows the study of structural changes that occur during an enzyme catalytic reaction. Serial synchrotron X-ray crystallography (SSX), which extends serial crystallography methods to synchrotron radiation sources, is expanding the scientific community using serial diffraction methods. This report presents a simple flow cell that can be used to deliver microcrystals across an X-ray beam during SSX studies. This device consists of an X-ray transparent glass capillary mounted on a goniometer-compatible 3D-printed support and is connected to a syringe pump via lightweight tubing. This flow cell is easily mounted and aligned, and it is disposable so can be rapidly replaced when blocked. This system was demonstrated by collecting SSX data at MAX IV Laboratory from microcrystals of the integral membrane protein cytochrome c oxidase from Thermus thermophilus, from which an X-ray structure was determined to 2.12 Å resolution. This simple SSX platform may help to lower entry barriers for non-expert users of SSX.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytochrome c oxidase, goniometer-compatible flow cells, macromolecular crystallography, serial synchrotron X-ray crystallography
in
Journal of Applied Crystallography
volume
56
issue
Pt 2
pages
12 pages
publisher
International Union of Crystallography
external identifiers
  • scopus:85159150434
  • pmid:37032973
ISSN
0021-8898
DOI
10.1107/S1600576723001036
language
English
LU publication?
yes
id
4abeff94-d997-4a6a-b014-08f713c4e215
date added to LUP
2023-08-14 08:26:26
date last changed
2025-04-20 10:48:31
@article{4abeff94-d997-4a6a-b014-08f713c4e215,
  abstract     = {{<p>Serial femtosecond crystallography was initially developed for room-temperature X-ray diffraction studies of macromolecules at X-ray free electron lasers. When combined with tools that initiate biological reactions within microcrystals, time-resolved serial crystallography allows the study of structural changes that occur during an enzyme catalytic reaction. Serial synchrotron X-ray crystallography (SSX), which extends serial crystallography methods to synchrotron radiation sources, is expanding the scientific community using serial diffraction methods. This report presents a simple flow cell that can be used to deliver microcrystals across an X-ray beam during SSX studies. This device consists of an X-ray transparent glass capillary mounted on a goniometer-compatible 3D-printed support and is connected to a syringe pump via lightweight tubing. This flow cell is easily mounted and aligned, and it is disposable so can be rapidly replaced when blocked. This system was demonstrated by collecting SSX data at MAX IV Laboratory from microcrystals of the integral membrane protein cytochrome c oxidase from Thermus thermophilus, from which an X-ray structure was determined to 2.12 Å resolution. This simple SSX platform may help to lower entry barriers for non-expert users of SSX.</p>}},
  author       = {{Ghosh, Swagatha and Zorić, Doris and Dahl, Peter and Bjelčić, Monika and Johannesson, Jonatan and Sandelin, Emil and Borjesson, Per and Björling, Alexander and Banacore, Analia and Edlund, Petra and Aurelius, Oskar and Milas, Mirko and Nan, Jie and Shilova, Anastasya and Gonzalez, Ana and Mueller, Uwe and Brändén, Gisela and Neutze, Richard}},
  issn         = {{0021-8898}},
  keywords     = {{cytochrome c oxidase; goniometer-compatible flow cells; macromolecular crystallography; serial synchrotron X-ray crystallography}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{Pt 2}},
  pages        = {{449--460}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Applied Crystallography}},
  title        = {{A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography}},
  url          = {{http://dx.doi.org/10.1107/S1600576723001036}},
  doi          = {{10.1107/S1600576723001036}},
  volume       = {{56}},
  year         = {{2023}},
}