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Laccase production by Galerina sp. and its application in dye decolorization

Mendoza, Laura LU ; Ibrahim, Victor LU ; Alvarez, Maria Teresa ; Hatti-Kaul, Rajni LU and Mamo, Gashaw LU (2014) In Journal of Yeast and Fungal Research 5(2). p.13-22
Abstract
The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly repressed by sinapyl alcohol, 3-(dimethylamino)benzoic acid, hydroquinone, 1-naphthale-neacetic acid, 2,6-dichlorophenol and 3-methyl-2-benzothiazolinone hydrazone. Cultivation under ... (More)
The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1 belongs to genus Galerina. High production of laccase was achieved in basal salt medium supplemented with 30 g L-1 glucose, 10 g L-1 yeast extract and 0.01 g L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly repressed by sinapyl alcohol, 3-(dimethylamino)benzoic acid, hydroquinone, 1-naphthale-neacetic acid, 2,6-dichlorophenol and 3-methyl-2-benzothiazolinone hydrazone. Cultivation under optimum conditions in the presence of 2 mmol L-1 2,5-xylidine and 0.01 g L-1 copper sulphate, resulted in the enzyme yield of over 26 000 U L-1. Galerina sp. HC1 could also produce laccase in media composed of orange peels both in submerged- and solid-state fermentations. The laccase (0.5 U ml-1) from Galerinasp. was able to decolorize over 60% of 35 μmol L-1 Congo red in 2 h in the presence of 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt as mediator, and 65% of 30 μmol L-1 Coomassie Brilliant Blue G-250 in 5 min using syringaldehyde as mediator. The laccase was immobilized on cross-linked chitosan and used efficiently to decolorize the dyes. (Less)
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Contribution to journal
publication status
published
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in
Journal of Yeast and Fungal Research
volume
5
issue
2
pages
13 - 22
DOI
10.5897/JYFR12.025
language
English
LU publication?
yes
id
4b06e6b5-0791-4e5b-ac37-3f5840998157
date added to LUP
2019-06-28 01:14:51
date last changed
2019-06-28 10:53:04
@article{4b06e6b5-0791-4e5b-ac37-3f5840998157,
  abstract     = {The laccase producing fungus HC1 was isolated from a sample collected in the Bolivian Amazon region. Analysis of 5.8S and 28S rDNA, and internal transcribed spacer 1 and 2 sequences revealed that isolate HC1  belongs  to  genus  Galerina.  High  production  of  laccase  was  achieved  in  basal  salt  medium  supplemented  with  30  g  L-1 glucose,  10  g  L-1  yeast  extract  and  0.01  g  L-1 copper sulphate. The enzyme production was further improved by addition of 2,5-xylidine, α-benzoin oxime and p-coumaric acid, but strongly  repressed  by  sinapyl  alcohol,  3-(dimethylamino)benzoic  acid,  hydroquinone,  1-naphthale-neacetic   acid,   2,6-dichlorophenol   and   3-methyl-2-benzothiazolinone   hydrazone.   Cultivation   under   optimum conditions in the presence of 2 mmol L-1 2,5-xylidine and 0.01 g L-1 copper sulphate, resulted in the enzyme yield of over 26 000 U L-1. Galerina sp. HC1 could also produce laccase in media composed of orange peels both in submerged- and solid-state fermentations. The laccase (0.5 U ml-1) from Galerinasp. was able to decolorize over 60% of 35 μmol L-1 Congo red in 2 h in the presence of 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt as mediator, and 65% of 30 μmol L-1 Coomassie Brilliant Blue G-250 in 5 min using syringaldehyde as mediator. The laccase was immobilized on cross-linked chitosan and used efficiently to decolorize the dyes.},
  author       = {Mendoza, Laura and Ibrahim, Victor and Alvarez, Maria Teresa and Hatti-Kaul, Rajni and Mamo, Gashaw},
  language     = {eng},
  number       = {2},
  pages        = {13--22},
  series       = {Journal of Yeast and Fungal Research},
  title        = {Laccase production by Galerina sp. and its application in dye decolorization},
  url          = {http://dx.doi.org/10.5897/JYFR12.025},
  doi          = {10.5897/JYFR12.025},
  volume       = {5},
  year         = {2014},
}