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Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent

Ekelund Ugge, Gustaf Mo LU orcid ; Jonsson, Annie and Berglund, Olof LU (2022) In Environmental Science and Pollution Research 29(2). p.2158-2170
Abstract

Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit... (More)

Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu> Ni > Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p > 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bivalve, Effect size, Mixture toxicity, RT-qPCR, Sex effects, Wastewater
in
Environmental Science and Pollution Research
volume
29
issue
2
pages
2158 - 2170
publisher
Springer
external identifiers
  • scopus:85112648604
  • pmid:34363176
ISSN
0944-1344
DOI
10.1007/s11356-021-15633-4
project
Transcriptional biomarkers of toxicity - powerful tools or random noise? An applied perspective from studies on bivalves
language
English
LU publication?
yes
id
4bd36b26-095b-46bd-a01d-992e38aaa21a
date added to LUP
2021-09-23 15:54:51
date last changed
2024-03-08 17:54:42
@article{4bd36b26-095b-46bd-a01d-992e38aaa21a,
  abstract     = {{<p>Using a selection of molecular biomarkers, we evaluated responses in freshwater mussels (Anodonta anatina) exposed to effluent from an industrial wastewater treatment facility. The aims of this work were to (1) assess biomarkers of general toxicity under sublethal exposure to an anthropogenic mixture of chemicals, represented by an arbitrary effluent, and (2) evaluate the potential of A. anatina as a bioindicator of pollution. Adult mussels (n = in total 32; 24 males and 8 females) were exposed (96 h) in the laboratory to a fixed dilution of effluent or to a control treatment of standardized freshwater. Metal concentrations were in general higher in the effluent, by an order of magnitude or more, compared to the control. Toxic unit estimates were used as proxies of chemical stress, and Cu, Ni, and Zn were identified as potential major contributors (Cu&gt; Ni &gt; Zn). Six transcriptional (cat, gst, hsp70, hsp90, mt, sod) and two biochemical (AChE, GST) biomarkers were analyzed in two tissues, gills, and digestive glands. Out of the 16 responses (eight biomarkers × two tissues), 14 effect sizes were small (within ± 28 % of control) and differences non-significant (p &gt; 0.05). Results did however show that (1) AChE activity increased by 40% in gills of exposed mussels compared to control, (2) hsp90 expression was 100% higher in exposed female gills compared to control, and (3) three marker signals (AChE in both tissues, and hsp70 in gills) differed between sexes, independent of treatment. Results highlight a need for further investigation of molecular biomarker variability and robustness in A. anatina.</p>}},
  author       = {{Ekelund Ugge, Gustaf Mo and Jonsson, Annie and Berglund, Olof}},
  issn         = {{0944-1344}},
  keywords     = {{Bivalve; Effect size; Mixture toxicity; RT-qPCR; Sex effects; Wastewater}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{2158--2170}},
  publisher    = {{Springer}},
  series       = {{Environmental Science and Pollution Research}},
  title        = {{Molecular biomarker responses in the freshwater mussel Anodonta anatina exposed to an industrial wastewater effluent}},
  url          = {{http://dx.doi.org/10.1007/s11356-021-15633-4}},
  doi          = {{10.1007/s11356-021-15633-4}},
  volume       = {{29}},
  year         = {{2022}},
}