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Potential Pitfalls of the Mx1-Cre System : Implications for Experimental Modeling of Normal and Malignant Hematopoiesis

Velasco, Talia LU ; Säwén, Petter LU ; Bryder, David LU and Cammenga, Jörg LU (2016) In Stem Cell Reports 7(1). p.11-18
Abstract

Conditional knockout mice are commonly used to study the function of specific genes in hematopoiesis. Different promoters that drive Cre expression have been utilized, with the interferon-inducible Mx1-Cre still being the most commonly used “deleter strain” in experimental hematology. However, different pitfalls associated with this system could lead to misinterpretation in functional studies. We present here two of these issues related to the use of Mx1-Cre: first, a high spontaneous recombination rate when applying commonly used techniques in experimental hematology, and second, undesired short-term consequences of the use of polyinosinic:polycytidylic acid, including changes in cellular phenotypes that, however, resolve within days.... (More)

Conditional knockout mice are commonly used to study the function of specific genes in hematopoiesis. Different promoters that drive Cre expression have been utilized, with the interferon-inducible Mx1-Cre still being the most commonly used “deleter strain” in experimental hematology. However, different pitfalls associated with this system could lead to misinterpretation in functional studies. We present here two of these issues related to the use of Mx1-Cre: first, a high spontaneous recombination rate when applying commonly used techniques in experimental hematology, and second, undesired short-term consequences of the use of polyinosinic:polycytidylic acid, including changes in cellular phenotypes that, however, resolve within days. Our studies emphasize therefore that proper controls are crucial when modeling gene deletion using the Mx1-Cre transgene.

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organization
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Contribution to journal
publication status
published
subject
in
Stem Cell Reports
volume
7
issue
1
pages
8 pages
publisher
Cell Press
external identifiers
  • scopus:84978531772
  • wos:000380492300003
ISSN
2213-6711
DOI
10.1016/j.stemcr.2016.06.002
language
English
LU publication?
yes
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4bf473d9-d8cd-4cb7-9531-9a38533695ee
date added to LUP
2016-12-22 08:49:09
date last changed
2017-11-12 04:27:31
@article{4bf473d9-d8cd-4cb7-9531-9a38533695ee,
  abstract     = {<p>Conditional knockout mice are commonly used to study the function of specific genes in hematopoiesis. Different promoters that drive Cre expression have been utilized, with the interferon-inducible Mx1-Cre still being the most commonly used “deleter strain” in experimental hematology. However, different pitfalls associated with this system could lead to misinterpretation in functional studies. We present here two of these issues related to the use of Mx1-Cre: first, a high spontaneous recombination rate when applying commonly used techniques in experimental hematology, and second, undesired short-term consequences of the use of polyinosinic:polycytidylic acid, including changes in cellular phenotypes that, however, resolve within days. Our studies emphasize therefore that proper controls are crucial when modeling gene deletion using the Mx1-Cre transgene.</p>},
  author       = {Velasco, Talia and Säwén, Petter and Bryder, David and Cammenga, Jörg},
  issn         = {2213-6711},
  language     = {eng},
  month        = {07},
  number       = {1},
  pages        = {11--18},
  publisher    = {Cell Press},
  series       = {Stem Cell Reports},
  title        = {Potential Pitfalls of the Mx1-Cre System : Implications for Experimental Modeling of Normal and Malignant Hematopoiesis},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2016.06.002},
  volume       = {7},
  year         = {2016},
}