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Small Alarmone Synthetases as novel bacterial RNA-binding proteins

Hauryliuk, Vasili LU orcid and Atkinson, Gemma C. LU (2017) In RNA Biology 14(12). p.1695-1699
Abstract

The alarmone nucleotides guanosine pentaphosphate (pppGpp) and tetraphosphate (ppGpp), collectively referred to as (p)ppGpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. We have recently shown that the Small Alarmone Synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis has an RNA-binding activity (Beljantseva et al. 2017). RelQ's activities as an enzyme and as an RNA-binding protein are mutually incompatible: binding of single-stranded RNA potently inhibits (p)ppGpp synthesis in a sequence-specific manner, and RelQ's enzymatic activity destabilizes the RNA:RelQ complex. RelQ's allosteric regulator, pppGpp, destabilizes RNA binding and activates RelQ's enzymatic... (More)

The alarmone nucleotides guanosine pentaphosphate (pppGpp) and tetraphosphate (ppGpp), collectively referred to as (p)ppGpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. We have recently shown that the Small Alarmone Synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis has an RNA-binding activity (Beljantseva et al. 2017). RelQ's activities as an enzyme and as an RNA-binding protein are mutually incompatible: binding of single-stranded RNA potently inhibits (p)ppGpp synthesis in a sequence-specific manner, and RelQ's enzymatic activity destabilizes the RNA:RelQ complex. RelQ's allosteric regulator, pppGpp, destabilizes RNA binding and activates RelQ's enzymatic activity. Since SAS enzymes are widely distributed in bacteria, and, as has been discovered recently, are also mobilized by phages (Dedrick et al. 2017), RNA binding to SASs could be a widespread mechanism. The initial discovery raises numerous questions regarding RNA-binding function of the SAS enzymes: What is the molecular mechanism underlying the incompatibility of RNA:SAS complex formation with pppGpp binding and (p)ppGpp synthesis? What are the RNA targets in living cells? What is the regulatory output of the system–(p)ppGpp synthesis, modulation of RNA structure and function, or both?.

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author
and
publishing date
type
Contribution to journal
publication status
published
keywords
CsrA, Hfq, nucleotide, ppGpp, RelQ, RNA, RNA-binding protein, RSH, SAS, stringent response
in
RNA Biology
volume
14
issue
12
pages
5 pages
publisher
Taylor & Francis
external identifiers
  • scopus:85038381901
  • pmid:28820325
ISSN
1547-6286
DOI
10.1080/15476286.2017.1367889
language
English
LU publication?
no
additional info
Funding Information: This work was supported by the Swedish Research Council (Veten-skapsra°det) under Grants 2013–4680 (VH) and 2015–04746 (GCA). Publisher Copyright: © 2017 Taylor & Francis Group, LLC. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
id
4c67cc0c-bad3-4e7a-9cb0-0e5447f6cb70
date added to LUP
2021-09-24 20:39:43
date last changed
2024-04-20 12:09:10
@article{4c67cc0c-bad3-4e7a-9cb0-0e5447f6cb70,
  abstract     = {{<p>The alarmone nucleotides guanosine pentaphosphate (pppGpp) and tetraphosphate (ppGpp), collectively referred to as (p)ppGpp, are key regulators of bacterial growth, stress adaptation, antibiotic tolerance and pathogenicity. We have recently shown that the Small Alarmone Synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis has an RNA-binding activity (Beljantseva et al. 2017). RelQ's activities as an enzyme and as an RNA-binding protein are mutually incompatible: binding of single-stranded RNA potently inhibits (p)ppGpp synthesis in a sequence-specific manner, and RelQ's enzymatic activity destabilizes the RNA:RelQ complex. RelQ's allosteric regulator, pppGpp, destabilizes RNA binding and activates RelQ's enzymatic activity. Since SAS enzymes are widely distributed in bacteria, and, as has been discovered recently, are also mobilized by phages (Dedrick et al. 2017), RNA binding to SASs could be a widespread mechanism. The initial discovery raises numerous questions regarding RNA-binding function of the SAS enzymes: What is the molecular mechanism underlying the incompatibility of RNA:SAS complex formation with pppGpp binding and (p)ppGpp synthesis? What are the RNA targets in living cells? What is the regulatory output of the system–(p)ppGpp synthesis, modulation of RNA structure and function, or both?.</p>}},
  author       = {{Hauryliuk, Vasili and Atkinson, Gemma C.}},
  issn         = {{1547-6286}},
  keywords     = {{CsrA; Hfq; nucleotide; ppGpp; RelQ; RNA; RNA-binding protein; RSH; SAS; stringent response}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{1695--1699}},
  publisher    = {{Taylor & Francis}},
  series       = {{RNA Biology}},
  title        = {{Small Alarmone Synthetases as novel bacterial RNA-binding proteins}},
  url          = {{http://dx.doi.org/10.1080/15476286.2017.1367889}},
  doi          = {{10.1080/15476286.2017.1367889}},
  volume       = {{14}},
  year         = {{2017}},
}