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Heparin-binding protein in lower airway samples as a biomarker for pneumonia

Paulsson, Magnus LU orcid ; Thelaus, Louise LU ; Riesbeck, Kristian LU orcid ; Qvarfordt, Ingemar ; Smith, Margaretha E. ; Lindén, Anders and Linder, Adam LU (2021) In Respiratory Research 22(1).
Abstract

Objectives: Ventilator-associated pneumonia (VAP) is difficult to diagnose using clinical criteria and no biomarkers have yet been proved to be sufficiently accurate. The use of the neutrophil-derived Heparin-binding protein (HBP) as a biomarker for pneumonia was investigated in this exploratory case–control study in two intensive care units at a tertiary referral hospital. Methods: Patients with clinical signs of pneumonia were recruited and bronchoalveolar lavage fluid (BALF) or bronchial wash (BW) samples were collected. Mechanically ventilated and lung healthy subjects were recruited as controls. HBP was measured with enzyme-linked immunosorbent assay. Results: BALF was collected from 14 patients with pneumonia and 14 healthy... (More)

Objectives: Ventilator-associated pneumonia (VAP) is difficult to diagnose using clinical criteria and no biomarkers have yet been proved to be sufficiently accurate. The use of the neutrophil-derived Heparin-binding protein (HBP) as a biomarker for pneumonia was investigated in this exploratory case–control study in two intensive care units at a tertiary referral hospital. Methods: Patients with clinical signs of pneumonia were recruited and bronchoalveolar lavage fluid (BALF) or bronchial wash (BW) samples were collected. Mechanically ventilated and lung healthy subjects were recruited as controls. HBP was measured with enzyme-linked immunosorbent assay. Results: BALF was collected from 14 patients with pneumonia and 14 healthy controls. Median HBP in BALF pneumonia samples was 14,690 ng/ml and controls 16.2 ng/ml (p < 0.0001). BW was collected from 10 pneumonia patients and 10 mechanically ventilated controls. Median HBP in BW pneumonia was 9002 ng/ml and controls 7.6 ng/ml (p < 0.0001). Conclusions: These data indicate that HBP concentrations is significantly higher in lower airway samples from patients with pneumonia than control subjects and is a potentially useful biomarker for diagnosis of VAP.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Assisted Ventilation, Biomarkers, Bronchoalveolar lavage, Critical Care, Neutrophil Biology, Pneumonia
in
Respiratory Research
volume
22
issue
1
article number
174
publisher
BioMed Central (BMC)
external identifiers
  • pmid:34103069
  • scopus:85107568114
ISSN
1465-9921
DOI
10.1186/s12931-021-01764-2
project
Diagnostic derivation and verification trial on biomarkers for ventilator-associated pneumonia
language
English
LU publication?
yes
id
4c907f6b-5ace-4b2c-8647-637b58c48b0c
date added to LUP
2021-07-01 14:02:49
date last changed
2024-04-20 08:06:14
@article{4c907f6b-5ace-4b2c-8647-637b58c48b0c,
  abstract     = {{<p>Objectives: Ventilator-associated pneumonia (VAP) is difficult to diagnose using clinical criteria and no biomarkers have yet been proved to be sufficiently accurate. The use of the neutrophil-derived Heparin-binding protein (HBP) as a biomarker for pneumonia was investigated in this exploratory case–control study in two intensive care units at a tertiary referral hospital. Methods: Patients with clinical signs of pneumonia were recruited and bronchoalveolar lavage fluid (BALF) or bronchial wash (BW) samples were collected. Mechanically ventilated and lung healthy subjects were recruited as controls. HBP was measured with enzyme-linked immunosorbent assay. Results: BALF was collected from 14 patients with pneumonia and 14 healthy controls. Median HBP in BALF pneumonia samples was 14,690 ng/ml and controls 16.2 ng/ml (p &lt; 0.0001). BW was collected from 10 pneumonia patients and 10 mechanically ventilated controls. Median HBP in BW pneumonia was 9002 ng/ml and controls 7.6 ng/ml (p &lt; 0.0001). Conclusions: These data indicate that HBP concentrations is significantly higher in lower airway samples from patients with pneumonia than control subjects and is a potentially useful biomarker for diagnosis of VAP.</p>}},
  author       = {{Paulsson, Magnus and Thelaus, Louise and Riesbeck, Kristian and Qvarfordt, Ingemar and Smith, Margaretha E. and Lindén, Anders and Linder, Adam}},
  issn         = {{1465-9921}},
  keywords     = {{Assisted Ventilation; Biomarkers; Bronchoalveolar lavage; Critical Care; Neutrophil Biology; Pneumonia}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Respiratory Research}},
  title        = {{Heparin-binding protein in lower airway samples as a biomarker for pneumonia}},
  url          = {{http://dx.doi.org/10.1186/s12931-021-01764-2}},
  doi          = {{10.1186/s12931-021-01764-2}},
  volume       = {{22}},
  year         = {{2021}},
}