PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera
Wahlberg, Niklas LU ; Peña, Carlos ; Ahola, Milla ; Wheat, Christopher W. and Rota, Jadranka LU
(2016)
In ZooKeys
2016(596).
p.129-141
- Abstract
We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4d9f721f-d8e5-4bcd-b9a6-2de8de0edb9e
- author
- Wahlberg, Niklas
LU
; Peña, Carlos
; Ahola, Milla
; Wheat, Christopher W.
and Rota, Jadranka
LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lepidoptera, Molecular systematics, Phylogenetics, Phylogenomics
- in
- ZooKeys
- volume
- 2016
- issue
- 596
- pages
- 13 pages
- publisher
- Pensoft Publishers
- external identifiers
-
- pmid:27408580
- wos:000378055700009
- scopus:84973367340
- ISSN
- 1313-2989
- DOI
- 10.3897/zookeys.596.8399
- language
- English
- LU publication?
- yes
- id
- 4d9f721f-d8e5-4bcd-b9a6-2de8de0edb9e
- date added to LUP
- 2016-09-08 12:10:46
- date last changed
- 2024-05-03 09:24:44
@article{4d9f721f-d8e5-4bcd-b9a6-2de8de0edb9e,
abstract = {{<p>We report primer pairs for 30 new gene regions in the nuclear genomes of Lepidoptera that can be amplified using a standard PCR protocol. The new primers were tested across diverse Lepidoptera, including nonditrysians and a wide selection of ditrysians. These new gene regions give a total of 11,043 bp of DNA sequence data and they show similar variability to traditionally used nuclear gene regions in studies of Lepidoptera. We feel that a PCR-based approach still has its place in molecular systematic studies of Lepidoptera, particularly at the intrafamilial level, and our new set of primers now provides a route to generating phylogenomic datasets using traditional methods.</p>}},
author = {{Wahlberg, Niklas and Peña, Carlos and Ahola, Milla and Wheat, Christopher W. and Rota, Jadranka}},
issn = {{1313-2989}},
keywords = {{Lepidoptera; Molecular systematics; Phylogenetics; Phylogenomics}},
language = {{eng}},
number = {{596}},
pages = {{129--141}},
publisher = {{Pensoft Publishers}},
series = {{ZooKeys}},
title = {{PCR primers for 30 novel gene regions in the nuclear genomes of Lepidoptera}},
url = {{http://dx.doi.org/10.3897/zookeys.596.8399}},
doi = {{10.3897/zookeys.596.8399}},
volume = {{2016}},
year = {{2016}},
}