A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.
(2000) In Journal of Nutritional Biochemistry 11(10). p.474-481- Abstract
- We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a... (More)
- We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a significantly (P = 0.001) lower level of membrane 70 & 75 kDa fraction and a higher (P = 0.001) level of cytosolic 40 & 43 kDa fraction than rats fed the casein diet. Because protein levels analyzed with a PKC ζ-specific antibody were similar, these differences in PKC ζ/λ were probably due to changes in PKC λ expression. PKC β2 levels did not differ between the dietary groups. Diet had no significant effect on the activity of acid, neutral, or alkaline sphingomyelinase. This study demonstrated that consumption of a high-beef diet is capable of modulating PKC isozyme levels in rat colon, which might be one of the mechanisms whereby red meat affects colon carcinogenesis. (Less)
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https://lup.lub.lu.se/record/4dc271e2-0277-487b-913c-1be82312ea9e
- author
- Pajari, Anne-Maria ; Oikarinen, SI ; Duan, Rui-Dong LU and Mutanen, Marja
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Nutritional Biochemistry
- volume
- 11
- issue
- 10
- pages
- 474 - 481
- publisher
- Elsevier
- external identifiers
-
- scopus:0033652746
- DOI
- 10.1016/S0955-2863(00)00100-5
- language
- English
- LU publication?
- no
- id
- 4dc271e2-0277-487b-913c-1be82312ea9e
- date added to LUP
- 2019-02-03 16:50:13
- date last changed
- 2022-01-31 17:21:55
@article{4dc271e2-0277-487b-913c-1be82312ea9e, abstract = {{We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a significantly (P = 0.001) lower level of membrane 70 & 75 kDa fraction and a higher (P = 0.001) level of cytosolic 40 & 43 kDa fraction than rats fed the casein diet. Because protein levels analyzed with a PKC ζ-specific antibody were similar, these differences in PKC ζ/λ were probably due to changes in PKC λ expression. PKC β2 levels did not differ between the dietary groups. Diet had no significant effect on the activity of acid, neutral, or alkaline sphingomyelinase. This study demonstrated that consumption of a high-beef diet is capable of modulating PKC isozyme levels in rat colon, which might be one of the mechanisms whereby red meat affects colon carcinogenesis.}}, author = {{Pajari, Anne-Maria and Oikarinen, SI and Duan, Rui-Dong and Mutanen, Marja}}, language = {{eng}}, number = {{10}}, pages = {{474--481}}, publisher = {{Elsevier}}, series = {{Journal of Nutritional Biochemistry}}, title = {{A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.}}, url = {{http://dx.doi.org/10.1016/S0955-2863(00)00100-5}}, doi = {{10.1016/S0955-2863(00)00100-5}}, volume = {{11}}, year = {{2000}}, }