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A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.

Pajari, Anne-Maria ; Oikarinen, SI ; Duan, Rui-Dong LU and Mutanen, Marja (2000) In Journal of Nutritional Biochemistry 11(10). p.474-481
Abstract
We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a... (More)
We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P < 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a significantly (P = 0.001) lower level of membrane 70 & 75 kDa fraction and a higher (P = 0.001) level of cytosolic 40 & 43 kDa fraction than rats fed the casein diet. Because protein levels analyzed with a PKC ζ-specific antibody were similar, these differences in PKC ζ/λ were probably due to changes in PKC λ expression. PKC β2 levels did not differ between the dietary groups. Diet had no significant effect on the activity of acid, neutral, or alkaline sphingomyelinase. This study demonstrated that consumption of a high-beef diet is capable of modulating PKC isozyme levels in rat colon, which might be one of the mechanisms whereby red meat affects colon carcinogenesis. (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
in
Journal of Nutritional Biochemistry
volume
11
issue
10
pages
474 - 481
publisher
Elsevier
external identifiers
  • scopus:0033652746
DOI
10.1016/S0955-2863(00)00100-5
language
English
LU publication?
no
id
4dc271e2-0277-487b-913c-1be82312ea9e
date added to LUP
2019-02-03 16:50:13
date last changed
2022-01-31 17:21:55
@article{4dc271e2-0277-487b-913c-1be82312ea9e,
  abstract     = {{We recently reported that a red meat (beef) diet relative to a casein-based diet increases protein kinase C (PKC) activity in rat colonic mucosa. The purpose of this study was to further elucidate the effects of a high-beef diet on colonic intracellular signal transduction by analyzing steady-state protein levels of different PKC isozymes as well as activities of the three types of sphingomyelinases. Male Wistar rats (n = 12/group) were fed AIN93G-based diets either high in beef or casein for 4 weeks. Rats fed the beef diet had significantly (P &lt; 0.05) higher cytosolic PKC α and lower membrane PKC δ protein levels than rats fed the casein diet. The beef-fed rats also had alterations in subfractions of PKC ζ/λ so that they had a significantly (P = 0.001) lower level of membrane 70 &amp; 75 kDa fraction and a higher (P = 0.001) level of cytosolic 40 &amp; 43 kDa fraction than rats fed the casein diet. Because protein levels analyzed with a PKC ζ-specific antibody were similar, these differences in PKC ζ/λ were probably due to changes in PKC λ expression. PKC β2 levels did not differ between the dietary groups. Diet had no significant effect on the activity of acid, neutral, or alkaline sphingomyelinase. This study demonstrated that consumption of a high-beef diet is capable of modulating PKC isozyme levels in rat colon, which might be one of the mechanisms whereby red meat affects colon carcinogenesis.}},
  author       = {{Pajari, Anne-Maria and Oikarinen, SI and Duan, Rui-Dong and Mutanen, Marja}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{474--481}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Nutritional Biochemistry}},
  title        = {{A high-beef diet alter protein kinase C isozyme expression in rat colonic mucosa.}},
  url          = {{http://dx.doi.org/10.1016/S0955-2863(00)00100-5}},
  doi          = {{10.1016/S0955-2863(00)00100-5}},
  volume       = {{11}},
  year         = {{2000}},
}