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Glial and neuronal marker proteins in the silicone chamber model for nerve regeneration

Karlsson, J E LU ; Rosengren, L E; Wang, S; Danielsen, N LU and Haglid, Kenneth G (1993) In Journal of Neurochemistry 60(3). p.1098-1104
Abstract

In the present study, neuronal and Schwann cell marker proteins were used to biochemically characterize the spatiotemporal progress of degeneration/regeneration in the silicone chamber model for nerve regeneration. Rat sciatic nerves were transected and the proximal and distal stumps were inserted into a bridging silicone chamber with a 10-mm interstump gap. Using dot immunobinding assays, S-100 protein and neuronal intermediate filament polypeptides were measured in different parts of the nerve 0-30 days after transection. In the most proximal nerve segment, all the measured proteins were transiently increased. In the proximal and distal stumps adjacent to the transection, the studied proteins were decreased indicating degeneration of... (More)

In the present study, neuronal and Schwann cell marker proteins were used to biochemically characterize the spatiotemporal progress of degeneration/regeneration in the silicone chamber model for nerve regeneration. Rat sciatic nerves were transected and the proximal and distal stumps were inserted into a bridging silicone chamber with a 10-mm interstump gap. Using dot immunobinding assays, S-100 protein and neuronal intermediate filament polypeptides were measured in different parts of the nerve 0-30 days after transection. In the most proximal nerve segment, all the measured proteins were transiently increased. In the proximal and distal stumps adjacent to the transection, the studied proteins were decreased indicating degeneration of the nerve. Within the silicone chamber, the regenerating nerve expressed the Schwann cell S-100 protein already at 7 days, whereas the neurofilament polypeptides appeared later. These observations are corroborated by previous morphological studies. The biochemical method described provides a new and fast approach to the study of nerve regeneration.

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published
subject
keywords
Animals, Biomarkers, Female, Nerve Regeneration, Nerve Tissue Proteins, Neurofilament Proteins, Neuroglia, Neurology, Neurons, Rats, Rats, Sprague-Dawley, S100 Proteins, Silicones, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Neurochemistry
volume
60
issue
3
pages
1098 - 1104
publisher
Wiley-Blackwell
external identifiers
  • scopus:0027400557
ISSN
0022-3042
DOI
10.1111/j.1471-4159.1993.tb03260.x
language
English
LU publication?
yes
id
4dc83786-3b5d-40f4-a540-c3d6c75802df
date added to LUP
2017-10-13 13:49:08
date last changed
2017-11-14 16:37:31
@article{4dc83786-3b5d-40f4-a540-c3d6c75802df,
  abstract     = {<p>In the present study, neuronal and Schwann cell marker proteins were used to biochemically characterize the spatiotemporal progress of degeneration/regeneration in the silicone chamber model for nerve regeneration. Rat sciatic nerves were transected and the proximal and distal stumps were inserted into a bridging silicone chamber with a 10-mm interstump gap. Using dot immunobinding assays, S-100 protein and neuronal intermediate filament polypeptides were measured in different parts of the nerve 0-30 days after transection. In the most proximal nerve segment, all the measured proteins were transiently increased. In the proximal and distal stumps adjacent to the transection, the studied proteins were decreased indicating degeneration of the nerve. Within the silicone chamber, the regenerating nerve expressed the Schwann cell S-100 protein already at 7 days, whereas the neurofilament polypeptides appeared later. These observations are corroborated by previous morphological studies. The biochemical method described provides a new and fast approach to the study of nerve regeneration.</p>},
  author       = {Karlsson, J E and Rosengren, L E and Wang, S and Danielsen, N and Haglid, Kenneth G},
  issn         = {0022-3042},
  keyword      = {Animals,Biomarkers,Female,Nerve Regeneration,Nerve Tissue Proteins,Neurofilament Proteins,Neuroglia,Neurology,Neurons,Rats,Rats, Sprague-Dawley,S100 Proteins,Silicones,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {1098--1104},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Glial and neuronal marker proteins in the silicone chamber model for nerve regeneration},
  url          = {http://dx.doi.org/10.1111/j.1471-4159.1993.tb03260.x},
  volume       = {60},
  year         = {1993},
}