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Identification and Functional Characterisation of Two Oat UDP-Glucosyltransferases Involved in Deoxynivalenol Detoxification

Khairullina, Alfia LU ; Renhuldt, Nikos Tsardakas LU orcid ; Wiesenberger, Gerlinde ; Bentzer, Johan LU ; Collinge, David B. ; Adam, Gerhard and Bülow, Leif LU (2022) In Toxins 14(7).
Abstract

Oat is susceptible to several Fusarium species that cause contamination with different trichothecene mycotoxins. The molecular mechanisms behind Fusarium resistance in oat have yet to be elucidated. In the present work, we identified and characterised two oat UDP-glucosyltransferases orthologous to barley HvUGT13248. Overexpression of the latter in wheat had been shown previously to increase resistance to deoxynivalenol (DON) and nivalenol (NIV) and to decrease disease the severity of both Fusarium head blight and Fusarium crown rot. Both oat genes are highly inducible by the application of DON and during infection with Fusarium graminearum. Heterologous expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae... (More)

Oat is susceptible to several Fusarium species that cause contamination with different trichothecene mycotoxins. The molecular mechanisms behind Fusarium resistance in oat have yet to be elucidated. In the present work, we identified and characterised two oat UDP-glucosyltransferases orthologous to barley HvUGT13248. Overexpression of the latter in wheat had been shown previously to increase resistance to deoxynivalenol (DON) and nivalenol (NIV) and to decrease disease the severity of both Fusarium head blight and Fusarium crown rot. Both oat genes are highly inducible by the application of DON and during infection with Fusarium graminearum. Heterologous expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae conferred high levels of resistance to DON, NIV and HT-2 toxins, but not C4-acetylated trichothecenes (T-2, diacetoxyscirpenol). Re-combinant enzymes AsUGT1 and AsUGT2 expressed in Escherichia coli rapidly lost activity upon purification, but the treatment of whole cells with the toxin clearly demonstrated the ability to convert DON into DON-3-O-glucoside. The two UGTs could therefore play an important role in counteracting the Fusarium virulence factor DON in oat.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
deoxynivalenol, glycosylation, oats, UDP-glucosyltransferase
in
Toxins
volume
14
issue
7
article number
446
publisher
MDPI AG
external identifiers
  • scopus:85133533650
  • pmid:35878183
ISSN
2072-6651
DOI
10.3390/toxins14070446
language
English
LU publication?
yes
id
4e2ffd7f-bda9-4512-a1e4-f861a64f87ec
date added to LUP
2022-09-22 15:25:48
date last changed
2024-06-13 19:34:32
@article{4e2ffd7f-bda9-4512-a1e4-f861a64f87ec,
  abstract     = {{<p>Oat is susceptible to several Fusarium species that cause contamination with different trichothecene mycotoxins. The molecular mechanisms behind Fusarium resistance in oat have yet to be elucidated. In the present work, we identified and characterised two oat UDP-glucosyltransferases orthologous to barley HvUGT13248. Overexpression of the latter in wheat had been shown previously to increase resistance to deoxynivalenol (DON) and nivalenol (NIV) and to decrease disease the severity of both Fusarium head blight and Fusarium crown rot. Both oat genes are highly inducible by the application of DON and during infection with Fusarium graminearum. Heterologous expression of these genes in a toxin-sensitive strain of Saccharomyces cerevisiae conferred high levels of resistance to DON, NIV and HT-2 toxins, but not C4-acetylated trichothecenes (T-2, diacetoxyscirpenol). Re-combinant enzymes AsUGT1 and AsUGT2 expressed in Escherichia coli rapidly lost activity upon purification, but the treatment of whole cells with the toxin clearly demonstrated the ability to convert DON into DON-3-O-glucoside. The two UGTs could therefore play an important role in counteracting the Fusarium virulence factor DON in oat.</p>}},
  author       = {{Khairullina, Alfia and Renhuldt, Nikos Tsardakas and Wiesenberger, Gerlinde and Bentzer, Johan and Collinge, David B. and Adam, Gerhard and Bülow, Leif}},
  issn         = {{2072-6651}},
  keywords     = {{deoxynivalenol; glycosylation; oats; UDP-glucosyltransferase}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{MDPI AG}},
  series       = {{Toxins}},
  title        = {{Identification and Functional Characterisation of Two Oat UDP-Glucosyltransferases Involved in Deoxynivalenol Detoxification}},
  url          = {{http://dx.doi.org/10.3390/toxins14070446}},
  doi          = {{10.3390/toxins14070446}},
  volume       = {{14}},
  year         = {{2022}},
}