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Influence of obestatin on the histological development of the small intestine in piglets during the first week of postnatal life

Woliński, J. LU ; Szczurek, P. LU ; Pierzynowska, K. LU orcid ; Wychowański, P. ; Seklecka, B. ; Boryczka, M. ; Kuwahara, A. ; Kato, I. ; Drahanchuk, O. LU and Zaworski, K. , et al. (2020) In Animal 14(10). p.2129-2137
Abstract

Obestatin is a gastrointestinal peptide having wide-ranging effects on cell proliferation; however, its mechanism of action remains poorly understood. Thus, the aim of the study was to elucidate the effect of exogenous obestatin on the postnatal structural development of the small intestine. Seven-day-old piglets with an average BW of 1.56 ± 0.23 kg were divided into four groups (n = 10) that received intragastrically obestatin (2, 10 or 15 μg/kg BW) or vehicle. After a 6-day experimental period, morphological analysis of gastrointestinal tract and small intestine wall (mitosis and apoptosis indexes, histomorphometry of mucosa and muscularis layers) was performed. The study revealed a seemingly incoherent pattern of the histological... (More)

Obestatin is a gastrointestinal peptide having wide-ranging effects on cell proliferation; however, its mechanism of action remains poorly understood. Thus, the aim of the study was to elucidate the effect of exogenous obestatin on the postnatal structural development of the small intestine. Seven-day-old piglets with an average BW of 1.56 ± 0.23 kg were divided into four groups (n = 10) that received intragastrically obestatin (2, 10 or 15 μg/kg BW) or vehicle. After a 6-day experimental period, morphological analysis of gastrointestinal tract and small intestine wall (mitosis and apoptosis indexes, histomorphometry of mucosa and muscularis layers) was performed. The study revealed a seemingly incoherent pattern of the histological structure of the small intestine among the experimental groups, suggesting that the effect of obestatin is both intestinal segment specific and dose dependent. Histomorphometric analysis of the small intestine showed that higher doses of obestatin seem to promote the structural development of the duodenum while simultaneously hindering the maturation of more distal parts of the intestine. Intragastric administration of obestatin increased the crypt mitotic index in all segments of the small intestine with the strongest pro-mitotic activity following the administration of obestatin at a dose of 10 and 15 μg/kg BW. The significant differences in the number of apoptotic cells in the intestinal villi among the groups were observed only in proximal jejunum and ileum. In conclusion, it seems that obestatin shows a broad-spectrum of activity in the gastrointestinal tract of newborn piglets, being able to accelerate its structural development. However, the varied effect depending on the intestinal segment or the concentration of exogenous obestatin causes that further research is needed to clarify the exact mechanism of this phenomenon.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enterocytes, intestinal mucosa, Keywords:, maturation, neonatal piglets, obestatin
in
Animal
volume
14
issue
10
pages
9 pages
publisher
Cambridge University Press
external identifiers
  • pmid:32398171
  • scopus:85085029967
ISSN
1751-7311
DOI
10.1017/S1751731120000919
language
English
LU publication?
yes
id
4e7ed2df-8835-4171-8252-b545d3738cae
date added to LUP
2020-06-18 12:29:47
date last changed
2024-06-26 18:14:18
@article{4e7ed2df-8835-4171-8252-b545d3738cae,
  abstract     = {{<p>Obestatin is a gastrointestinal peptide having wide-ranging effects on cell proliferation; however, its mechanism of action remains poorly understood. Thus, the aim of the study was to elucidate the effect of exogenous obestatin on the postnatal structural development of the small intestine. Seven-day-old piglets with an average BW of 1.56 ± 0.23 kg were divided into four groups (n = 10) that received intragastrically obestatin (2, 10 or 15 μg/kg BW) or vehicle. After a 6-day experimental period, morphological analysis of gastrointestinal tract and small intestine wall (mitosis and apoptosis indexes, histomorphometry of mucosa and muscularis layers) was performed. The study revealed a seemingly incoherent pattern of the histological structure of the small intestine among the experimental groups, suggesting that the effect of obestatin is both intestinal segment specific and dose dependent. Histomorphometric analysis of the small intestine showed that higher doses of obestatin seem to promote the structural development of the duodenum while simultaneously hindering the maturation of more distal parts of the intestine. Intragastric administration of obestatin increased the crypt mitotic index in all segments of the small intestine with the strongest pro-mitotic activity following the administration of obestatin at a dose of 10 and 15 μg/kg BW. The significant differences in the number of apoptotic cells in the intestinal villi among the groups were observed only in proximal jejunum and ileum. In conclusion, it seems that obestatin shows a broad-spectrum of activity in the gastrointestinal tract of newborn piglets, being able to accelerate its structural development. However, the varied effect depending on the intestinal segment or the concentration of exogenous obestatin causes that further research is needed to clarify the exact mechanism of this phenomenon.</p>}},
  author       = {{Woliński, J. and Szczurek, P. and Pierzynowska, K. and Wychowański, P. and Seklecka, B. and Boryczka, M. and Kuwahara, A. and Kato, I. and Drahanchuk, O. and Zaworski, K. and Pierzynowski, S. G. and Słupecka-Ziemilska, M.}},
  issn         = {{1751-7311}},
  keywords     = {{enterocytes; intestinal mucosa; Keywords:; maturation; neonatal piglets; obestatin}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{10}},
  pages        = {{2129--2137}},
  publisher    = {{Cambridge University Press}},
  series       = {{Animal}},
  title        = {{Influence of obestatin on the histological development of the small intestine in piglets during the first week of postnatal life}},
  url          = {{http://dx.doi.org/10.1017/S1751731120000919}},
  doi          = {{10.1017/S1751731120000919}},
  volume       = {{14}},
  year         = {{2020}},
}