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Localization of protein gene product 9.5 immunoreactivity in derivatives of the human Wolffian duct and in prostate cancer

Aumüller, G. ; Renneberg, H. ; Leonhardt, M. ; Lilja, H. LU orcid and Abrahamsson, Per Anders LU (1999) In Prostate 38(4). p.261-267
Abstract

BACKGROUND. Protein gene product 9.5 (PGP 9.5) has been considered to be a neuronal marker, but it is also present in extraneuronal tissues, e.g., the human mammary gland and rat epididymis. Its presence and distribution in the developing and adult male human genital tract have been unknown. METHODS. Immunohistochemical reactions were performed on human embryonic and postnatal specimens of the male genital tract, using commercial monoclonal and polyclonal antibodies. RESULTS. PGP 9.5 immunoreactivity was found in the Wolffian duct of human embryos (55-85 mm crown-rump length). Strong reactivity was observed in mesonephric tubular cells and at the apical rim of Wolffian duct cells. Owing to their PGP 9.5 immunoreactivity, these cells... (More)

BACKGROUND. Protein gene product 9.5 (PGP 9.5) has been considered to be a neuronal marker, but it is also present in extraneuronal tissues, e.g., the human mammary gland and rat epididymis. Its presence and distribution in the developing and adult male human genital tract have been unknown. METHODS. Immunohistochemical reactions were performed on human embryonic and postnatal specimens of the male genital tract, using commercial monoclonal and polyclonal antibodies. RESULTS. PGP 9.5 immunoreactivity was found in the Wolffian duct of human embryos (55-85 mm crown-rump length). Strong reactivity was observed in mesonephric tubular cells and at the apical rim of Wolffian duct cells. Owing to their PGP 9.5 immunoreactivity, these cells could also be identified on the surface of the embryonic verumontanum, extending from the orifices of the Wolffian duct to a small stretch of the urogenital sinus. There they contrasted sharply against non-Wolffian cells. In the adult human genital tract, PGP 9.5 immunoreactive material was present in the supranuclear portion of some epithelial cells of the epididymal efferent ductules, in isolated cells of the ejaculatory ducts, and in prostate cancer specimens. In the ejaculatory ducts, the PGP 9.5- immunoreactive cells were free of immunoreactivity for semenogelin, the major secretory product of the ejaculatory-vesicular-ampullary complex, and they also lacked chromogranin A-immunoreactivity. In prostate cancer specimens, PGP 9.5 immunoreactivity was never observed in secretory cells (immunoreactive for prostate-specific antigen), but was restricted to neuroendocrine cells, where it occurred either alone or coexpressed with chromogranin A-immunoreactivity. CONCLUSIONS. PGP 9.5-immunoreactivity is prenatally distributed in the Wolffian duct and its derivations; postnatally, it is restricted to a few cells derived from the initial and terminal segment of the Wolffian duct, and to neuroendocrine cells in prostate cancer specimens.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Human male genital system, Immunohistochemistry, Prostatic cancer, Protein gene product 9.5, Wolffian duct
in
Prostate
volume
38
issue
4
pages
261 - 267
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:10075005
  • scopus:0344527921
ISSN
0270-4137
DOI
10.1002/(SICI)1097-0045(19990301)38:4<261::AID-PROS1>3.3.CO;2-5
language
English
LU publication?
yes
id
4eb1c258-f677-4d44-bd89-5e7d79623c6b
date added to LUP
2022-12-06 17:41:39
date last changed
2024-01-13 13:35:27
@article{4eb1c258-f677-4d44-bd89-5e7d79623c6b,
  abstract     = {{<p>BACKGROUND. Protein gene product 9.5 (PGP 9.5) has been considered to be a neuronal marker, but it is also present in extraneuronal tissues, e.g., the human mammary gland and rat epididymis. Its presence and distribution in the developing and adult male human genital tract have been unknown. METHODS. Immunohistochemical reactions were performed on human embryonic and postnatal specimens of the male genital tract, using commercial monoclonal and polyclonal antibodies. RESULTS. PGP 9.5 immunoreactivity was found in the Wolffian duct of human embryos (55-85 mm crown-rump length). Strong reactivity was observed in mesonephric tubular cells and at the apical rim of Wolffian duct cells. Owing to their PGP 9.5 immunoreactivity, these cells could also be identified on the surface of the embryonic verumontanum, extending from the orifices of the Wolffian duct to a small stretch of the urogenital sinus. There they contrasted sharply against non-Wolffian cells. In the adult human genital tract, PGP 9.5 immunoreactive material was present in the supranuclear portion of some epithelial cells of the epididymal efferent ductules, in isolated cells of the ejaculatory ducts, and in prostate cancer specimens. In the ejaculatory ducts, the PGP 9.5- immunoreactive cells were free of immunoreactivity for semenogelin, the major secretory product of the ejaculatory-vesicular-ampullary complex, and they also lacked chromogranin A-immunoreactivity. In prostate cancer specimens, PGP 9.5 immunoreactivity was never observed in secretory cells (immunoreactive for prostate-specific antigen), but was restricted to neuroendocrine cells, where it occurred either alone or coexpressed with chromogranin A-immunoreactivity. CONCLUSIONS. PGP 9.5-immunoreactivity is prenatally distributed in the Wolffian duct and its derivations; postnatally, it is restricted to a few cells derived from the initial and terminal segment of the Wolffian duct, and to neuroendocrine cells in prostate cancer specimens.</p>}},
  author       = {{Aumüller, G. and Renneberg, H. and Leonhardt, M. and Lilja, H. and Abrahamsson, Per Anders}},
  issn         = {{0270-4137}},
  keywords     = {{Human male genital system; Immunohistochemistry; Prostatic cancer; Protein gene product 9.5; Wolffian duct}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{4}},
  pages        = {{261--267}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Prostate}},
  title        = {{Localization of protein gene product 9.5 immunoreactivity in derivatives of the human Wolffian duct and in prostate cancer}},
  url          = {{http://dx.doi.org/10.1002/(SICI)1097-0045(19990301)38:4<261::AID-PROS1>3.3.CO;2-5}},
  doi          = {{10.1002/(SICI)1097-0045(19990301)38:4<261::AID-PROS1>3.3.CO;2-5}},
  volume       = {{38}},
  year         = {{1999}},
}