Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Diversity of human papillomaviruses in skin lesions.

Ekström, Johanna LU ; Mühr, Laila Sara Arroyo ; Bzhalava, Davit LU ; Söderlund Strand, Anna LU ; Hultin, Emilie ; Nordin, Peter ; Stenquist, Bo ; Paoli, John ; Forslund, Ola LU and Dillner, Joakim LU (2013) In Virology 447(1-2). p.300-311
Abstract
Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for... (More)
Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virology
volume
447
issue
1-2
pages
300 - 311
publisher
Elsevier
external identifiers
  • wos:000326553500034
  • pmid:24210127
  • scopus:84885334193
  • pmid:24210127
ISSN
1096-0341
DOI
10.1016/j.virol.2013.09.010
language
English
LU publication?
yes
id
4eb82c77-c1e2-4962-bec9-203d46a763a2 (old id 4179614)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24210127?dopt=Abstract
date added to LUP
2016-04-01 10:16:23
date last changed
2022-03-19 19:08:20
@article{4eb82c77-c1e2-4962-bec9-203d46a763a2,
  abstract     = {{Pools of frozen biopsies from patients with squamous cell carcinoma (SCC) (n=29) actinic keratosis (AK) (n=31), keratoacanthoma (n=91) and swab samples from 84 SCCs and 91 AKs were analysed with an extended HPV general primer PCR and high-throughput sequencing of amplimers. We found 273 different HPV isolates (87 known HPV types, 139 previously known HPV sequences (putative types) and 47 sequences from novel putative HPV types). Among the new sequences, five clustered in genus Betapapillomavirus and 42 in genus Gammapapillomavirus. Resequencing of the three pools between 21 to 70 times resulted in the detection of 283 different known or putative HPV types, with 156 different sequences found in only one of the pools. Type-specific PCRs for 37 putative types from an additional 296 patients found only two of these putative types. In conclusion, skin lesions contain a large diversity of HPV types, but most appeared to be rare infections.}},
  author       = {{Ekström, Johanna and Mühr, Laila Sara Arroyo and Bzhalava, Davit and Söderlund Strand, Anna and Hultin, Emilie and Nordin, Peter and Stenquist, Bo and Paoli, John and Forslund, Ola and Dillner, Joakim}},
  issn         = {{1096-0341}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{300--311}},
  publisher    = {{Elsevier}},
  series       = {{Virology}},
  title        = {{Diversity of human papillomaviruses in skin lesions.}},
  url          = {{http://dx.doi.org/10.1016/j.virol.2013.09.010}},
  doi          = {{10.1016/j.virol.2013.09.010}},
  volume       = {{447}},
  year         = {{2013}},
}