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Odontoblast-like MDPC-23 cells produce pro-inflammatory IL-6 in response to lipoteichoic acid and express the antimicrobial peptide CRAMP

Odlén, Karin ; Fält, Felicia ; Dahl, Sara LU orcid ; Aidoukovitch, Alexandra LU ; Ericson, Dan ; Nilsson, Bengt Olof LU orcid and Hedenbjörk-Lager, Anders (2020) In Acta Odontologica Scandinavica 78(3). p.210-216
Abstract

Objective: Odontoblasts are thought to be involved in innate immunity but their precise role in this process is not fully understood. Here, we assess effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA), produced by Gram-negative and Gram-positive bacteria, respectively, on matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and cathelin-related antimicrobial peptide (CRAMP) expression in odontoblast-like MDPC-23 cells. Material and methods: Gene activity and protein production was determined by quantitative real-time RT-PCR and ELISA, respectively. Cellular expression of CRAMP was determined by immunocytochemistry. Results: Stimulation with LTA (5 and 25 µg/ml) but not LPS (1 and 5 µg/ml) for 24 h enhanced IL-6 mRNA... (More)

Objective: Odontoblasts are thought to be involved in innate immunity but their precise role in this process is not fully understood. Here, we assess effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA), produced by Gram-negative and Gram-positive bacteria, respectively, on matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and cathelin-related antimicrobial peptide (CRAMP) expression in odontoblast-like MDPC-23 cells. Material and methods: Gene activity and protein production was determined by quantitative real-time RT-PCR and ELISA, respectively. Cellular expression of CRAMP was determined by immunocytochemistry. Results: Stimulation with LTA (5 and 25 µg/ml) but not LPS (1 and 5 µg/ml) for 24 h enhanced IL-6 mRNA expression. The LTA-induced up-regulation of IL-6 mRNA levels was associated with increased IL-6 protein levels. Stimulation with either LPS or LTA for 24 h lacked effect on both MMP-8 transcript and protein expression. Immunocytochemistry disclosed that MDPC-23 cells expressed immunoreactivity for CRAMP. MDPC-23 cells showed mRNA expression for CRAMP, but stimulation with either LPS or LTA did not modulate CRAMP transcript expression. Conclusions: We show that MDPC-23 cells possess immune-like cell properties such as LTA-induced IL-6 production and expression of the antimicrobial peptide CRAMP, suggesting that odontoblasts may modulate innate immunity via these mechanisms.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antimicrobial peptides, cytokine, innate immunity, odontoblast
in
Acta Odontologica Scandinavica
volume
78
issue
3
pages
7 pages
publisher
Taylor & Francis
external identifiers
  • scopus:85075212946
  • pmid:31726911
ISSN
0001-6357
DOI
10.1080/00016357.2019.1685679
project
Production and transport of the antimicrobial peptide LL-37 and other peptides in saliva: physiological and pathophysiological importance
language
English
LU publication?
yes
id
4f2a2801-f8fd-4b36-8e07-3eed45553cd0
date added to LUP
2019-12-06 13:22:15
date last changed
2024-04-02 22:51:23
@article{4f2a2801-f8fd-4b36-8e07-3eed45553cd0,
  abstract     = {{<p>Objective: Odontoblasts are thought to be involved in innate immunity but their precise role in this process is not fully understood. Here, we assess effects of lipopolysaccharide (LPS) and lipoteichoic acid (LTA), produced by Gram-negative and Gram-positive bacteria, respectively, on matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and cathelin-related antimicrobial peptide (CRAMP) expression in odontoblast-like MDPC-23 cells. Material and methods: Gene activity and protein production was determined by quantitative real-time RT-PCR and ELISA, respectively. Cellular expression of CRAMP was determined by immunocytochemistry. Results: Stimulation with LTA (5 and 25 µg/ml) but not LPS (1 and 5 µg/ml) for 24 h enhanced IL-6 mRNA expression. The LTA-induced up-regulation of IL-6 mRNA levels was associated with increased IL-6 protein levels. Stimulation with either LPS or LTA for 24 h lacked effect on both MMP-8 transcript and protein expression. Immunocytochemistry disclosed that MDPC-23 cells expressed immunoreactivity for CRAMP. MDPC-23 cells showed mRNA expression for CRAMP, but stimulation with either LPS or LTA did not modulate CRAMP transcript expression. Conclusions: We show that MDPC-23 cells possess immune-like cell properties such as LTA-induced IL-6 production and expression of the antimicrobial peptide CRAMP, suggesting that odontoblasts may modulate innate immunity via these mechanisms.</p>}},
  author       = {{Odlén, Karin and Fält, Felicia and Dahl, Sara and Aidoukovitch, Alexandra and Ericson, Dan and Nilsson, Bengt Olof and Hedenbjörk-Lager, Anders}},
  issn         = {{0001-6357}},
  keywords     = {{Antimicrobial peptides; cytokine; innate immunity; odontoblast}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{210--216}},
  publisher    = {{Taylor & Francis}},
  series       = {{Acta Odontologica Scandinavica}},
  title        = {{Odontoblast-like MDPC-23 cells produce pro-inflammatory IL-6 in response to lipoteichoic acid and express the antimicrobial peptide CRAMP}},
  url          = {{http://dx.doi.org/10.1080/00016357.2019.1685679}},
  doi          = {{10.1080/00016357.2019.1685679}},
  volume       = {{78}},
  year         = {{2020}},
}