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Different patterns of clonal evolution among different sarcoma subtypes followed for up to 25 years

Hofvander, Jakob LU ; Viklund, Björn; Isaksson, Anders; Brosjö, Otte; Vult von Steyern, Fredrik LU ; Rissler, Pehr LU ; Mandahl, Nils LU and Mertens, Fredrik LU (2018) In Nature Communications 9(1).
Abstract

To compare clonal evolution in tumors arising through different mechanisms, we selected three types of sarcoma—amplicon-driven well-differentiated liposarcoma (WDLS), gene fusion-driven myxoid liposarcoma (MLS), and sarcomas with complex genomes (CXS)—and assessed the dynamics of chromosome and nucleotide level mutations by cytogenetics, SNP array analysis and whole-exome sequencing. Here we show that the extensive single-cell variation in WDLS has minor impact on clonal key amplicons in chromosome 12. In addition, only a few of the single nucleotide variants in WDLS were present in more than one lesion, suggesting that such mutations are of little significance in tumor development. MLS displays few mutations other than the FUS-DDIT3... (More)

To compare clonal evolution in tumors arising through different mechanisms, we selected three types of sarcoma—amplicon-driven well-differentiated liposarcoma (WDLS), gene fusion-driven myxoid liposarcoma (MLS), and sarcomas with complex genomes (CXS)—and assessed the dynamics of chromosome and nucleotide level mutations by cytogenetics, SNP array analysis and whole-exome sequencing. Here we show that the extensive single-cell variation in WDLS has minor impact on clonal key amplicons in chromosome 12. In addition, only a few of the single nucleotide variants in WDLS were present in more than one lesion, suggesting that such mutations are of little significance in tumor development. MLS displays few mutations other than the FUS-DDIT3 fusion, and the primary tumor is genetically sometimes much more complex than its relapses, whereas CXS in general shows a gradual increase of both nucleotide- and chromosome–level mutations, similar to what has been described in carcinomas.

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organization
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Contribution to journal
publication status
published
subject
in
Nature Communications
volume
9
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85053222200
ISSN
2041-1723
DOI
10.1038/s41467-018-06098-0
language
English
LU publication?
yes
id
4f599709-df21-4e83-bf75-94f1d5b3d01f
date added to LUP
2018-10-08 11:40:24
date last changed
2019-01-14 07:38:52
@article{4f599709-df21-4e83-bf75-94f1d5b3d01f,
  abstract     = {<p>To compare clonal evolution in tumors arising through different mechanisms, we selected three types of sarcoma—amplicon-driven well-differentiated liposarcoma (WDLS), gene fusion-driven myxoid liposarcoma (MLS), and sarcomas with complex genomes (CXS)—and assessed the dynamics of chromosome and nucleotide level mutations by cytogenetics, SNP array analysis and whole-exome sequencing. Here we show that the extensive single-cell variation in WDLS has minor impact on clonal key amplicons in chromosome 12. In addition, only a few of the single nucleotide variants in WDLS were present in more than one lesion, suggesting that such mutations are of little significance in tumor development. MLS displays few mutations other than the FUS-DDIT3 fusion, and the primary tumor is genetically sometimes much more complex than its relapses, whereas CXS in general shows a gradual increase of both nucleotide- and chromosome–level mutations, similar to what has been described in carcinomas.</p>},
  articleno    = {3662},
  author       = {Hofvander, Jakob and Viklund, Björn and Isaksson, Anders and Brosjö, Otte and Vult von Steyern, Fredrik and Rissler, Pehr and Mandahl, Nils and Mertens, Fredrik},
  issn         = {2041-1723},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Different patterns of clonal evolution among different sarcoma subtypes followed for up to 25 years},
  url          = {http://dx.doi.org/10.1038/s41467-018-06098-0},
  volume       = {9},
  year         = {2018},
}