Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
(2023) In European Journal of Immunology 53(11).- Abstract
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney,... (More)
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.
(Less)
- author
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dendritic cells, Discrimination of dendritic cell subsets, Mouse nonlymphoid tissue, Multiplex flow cytometry analysis, Tissue digestion protocols
- in
- European Journal of Immunology
- volume
- 53
- issue
- 11
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:36512638
- scopus:85144063646
- ISSN
- 0014-2980
- DOI
- 10.1002/eji.202249819
- language
- English
- LU publication?
- yes
- id
- 4fbd1159-6434-4854-9e92-9d2faf15c531
- date added to LUP
- 2023-01-25 15:51:12
- date last changed
- 2024-04-18 08:46:19
@article{4fbd1159-6434-4854-9e92-9d2faf15c531,
abstract = {{<p>This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various nonlymphoid tissues. DC are sentinels of the immune system present in almost every mammalian organ. Since they represent a rare cell population, DC need to be extracted from organs with protocols that are specifically developed for each tissue. This article provides detailed protocols for the preparation of single-cell suspensions from various mouse nonlymphoid tissues, including skin, intestine, lung, kidney, mammary glands, oral mucosa and transplantable tumors. Furthermore, our guidelines include comprehensive protocols for multiplex flow cytometry analysis of DC subsets and feature top tricks for their proper discrimination from other myeloid cells. With this collection, we provide guidelines for in-depth analysis of DC subsets that will advance our understanding of their respective roles in healthy and diseased tissues. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all coauthors, making it an essential resource for basic and clinical DC immunologists.</p>}},
author = {{Probst, Hans Christian and Stoitzner, Patrizia and Amon, Lukas and Backer, Ronald A. and Brand, Anna and Chen, Jianzhou and Clausen, Björn E. and Dieckmann, Sophie and Dudziak, Diana and Heger, Lukas and Hodapp, Katrin and Hornsteiner, Florian and Hovav, Avi Hai and Jacobi, Lukas and Ji, Xingqi and Kamenjarin, Nadine and Lahl, Katharina and Lahmar, Imran and Lakus, Jelena and Lehmann, Christian H.K. and Ortner, Daniela and Picard, Marion and Roberti, Maria Paula and Rossnagel, Lukas and Saba, Yasmin and Schalla, Carmen and Schlitzer, Andreas and Schraml, Barbara U. and Schütze, Kristian and Seichter, Anna and Seré, Kristin and Seretis, Athanasios and Sopper, Sieghart and Strandt, Helen and Sykora, Martina M. and Theobald, Hannah and Tripp, Christoph H. and Zitvogel, Laurence}},
issn = {{0014-2980}},
keywords = {{Dendritic cells; Discrimination of dendritic cell subsets; Mouse nonlymphoid tissue; Multiplex flow cytometry analysis; Tissue digestion protocols}},
language = {{eng}},
number = {{11}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues}},
url = {{http://dx.doi.org/10.1002/eji.202249819}},
doi = {{10.1002/eji.202249819}},
volume = {{53}},
year = {{2023}},
}