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Pigment composition and photoacclimation as keys to the ecological success of Gonyostomum semen (Raphidophyceae, Stramenopiles)

Sassenhagen, Ingrid LU ; Rengefors, Karin LU ; Richardson, Tammi L. and Pinckney, James L. (2014) In Journal of Phycology 50(6). p.1146-1154
Abstract
Aquatic habitats are usually structured by light attenuation with depth resulting in different microalgal communities, each one adapted to a certain light regime by their specific pigment composition. Several taxa contain pigments restricted to one phylogenetic group, making them useful as marker pigments in phytoplankton community studies. The nuisance and invasive freshwater microalga Gonyostomum semen (Ehrenberg) (Raphidophyceae) is mainly found in brown water lakes with sharp vertical gradients in light intensity and color. However, its pigment composition and potential photoadaptations have not been comprehensively studied. We analyzed the photopigment composition of 12 genetically different strains of G. semen by HPLC after... (More)
Aquatic habitats are usually structured by light attenuation with depth resulting in different microalgal communities, each one adapted to a certain light regime by their specific pigment composition. Several taxa contain pigments restricted to one phylogenetic group, making them useful as marker pigments in phytoplankton community studies. The nuisance and invasive freshwater microalga Gonyostomum semen (Ehrenberg) (Raphidophyceae) is mainly found in brown water lakes with sharp vertical gradients in light intensity and color. However, its pigment composition and potential photoadaptations have not been comprehensively studied. We analyzed the photopigment composition of 12 genetically different strains of G. semen by HPLC after acclimation to different light conditions. We confirmed the pigments chlorophyll a, chlorophyll c1c2, diadinoxanthin, trans-neoxanthin, cis-neoxanthin, α and β carotene, which have already been reported for G. semen. Additionally, we identified, for the first time, the pigments violaxanthin, zeaxanthin and alloxanthin in this species. Alloxanthin has never been observed in raphidophytes before, suggesting differences in evolutionary plastid acquisition between freshwater lineages and the well-described marine species. The amount of total chlorophyll a per cell generally decreased with increasing light intensity. In contrast, the increasing ratios of the prominent pigments diadinoxanthin and alloxanthin per chlorophyll a with light intensity suggest photoprotective functions. Additionally we found significant variation in cell-specific pigment concentration among strains, grouped by lake of origin, which might correspond to genetic differences between strains and populations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alloxanthin, Gonyostomum semen, HPLC, photoacclimation, pigments, raphidophyte
in
Journal of Phycology
volume
50
issue
6
pages
1146 - 1154
publisher
Wiley-Blackwell
external identifiers
  • wos:000345760300016
  • scopus:84912574255
ISSN
0022-3646
DOI
10.1111/jpy.12246
project
CAnMove
language
English
LU publication?
yes
id
19b2c510-c4ec-460c-a2a5-9704362ec90d (old id 5010673)
alternative location
http://dx.doi.org/10.1111/jpy.12246
date added to LUP
2015-01-30 14:43:41
date last changed
2017-01-01 04:03:01
@article{19b2c510-c4ec-460c-a2a5-9704362ec90d,
  abstract     = {Aquatic habitats are usually structured by light attenuation with depth resulting in different microalgal communities, each one adapted to a certain light regime by their specific pigment composition. Several taxa contain pigments restricted to one phylogenetic group, making them useful as marker pigments in phytoplankton community studies. The nuisance and invasive freshwater microalga Gonyostomum semen (Ehrenberg) (Raphidophyceae) is mainly found in brown water lakes with sharp vertical gradients in light intensity and color. However, its pigment composition and potential photoadaptations have not been comprehensively studied. We analyzed the photopigment composition of 12 genetically different strains of G. semen by HPLC after acclimation to different light conditions. We confirmed the pigments chlorophyll a, chlorophyll c1c2, diadinoxanthin, trans-neoxanthin, cis-neoxanthin, α and β carotene, which have already been reported for G. semen. Additionally, we identified, for the first time, the pigments violaxanthin, zeaxanthin and alloxanthin in this species. Alloxanthin has never been observed in raphidophytes before, suggesting differences in evolutionary plastid acquisition between freshwater lineages and the well-described marine species. The amount of total chlorophyll a per cell generally decreased with increasing light intensity. In contrast, the increasing ratios of the prominent pigments diadinoxanthin and alloxanthin per chlorophyll a with light intensity suggest photoprotective functions. Additionally we found significant variation in cell-specific pigment concentration among strains, grouped by lake of origin, which might correspond to genetic differences between strains and populations.},
  author       = {Sassenhagen, Ingrid and Rengefors, Karin and Richardson, Tammi L. and Pinckney, James L.},
  issn         = {0022-3646},
  keyword      = {alloxanthin,Gonyostomum semen,HPLC,photoacclimation,pigments,raphidophyte},
  language     = {eng},
  number       = {6},
  pages        = {1146--1154},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Phycology},
  title        = {Pigment composition and photoacclimation as keys to the ecological success of Gonyostomum semen (Raphidophyceae, Stramenopiles)},
  url          = {http://dx.doi.org/10.1111/jpy.12246},
  volume       = {50},
  year         = {2014},
}