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Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase

Faryar, Reza LU ; Linares-Pastén, Javier LU ; Immerzeel, Peter LU ; Mamo, Gashaw LU ; Andersson, Maria LU ; Stålbrand, Henrik LU ; Mattiasson, Bo LU and Nordberg Karlsson, Eva LU (2015) In Food and Bioproducts Processing 93(Online 22 November 2014). p.1-10
Abstract
Agricultural by-products are raw materials of importance for increased utilization of renewable biomass. Wheat straw is a raw material of significant production volume and is in this work used for production of xylooligosaccharides (XOS). Extraction of xylan by dilute alkali was followed by hydrolysis using a variant of the alkali-tolerant Bacillus halodurans S7 endoxylanase A mutated at K80R. The xylan yield was on average 56.5 g xylose equivalents per kg dried, ground wheat straw, with 1 arabinose per 12 xylose residues. The K80R variant, which displayed higher specific activity than the wild-type enzyme, was added at a load of 96 U/g extracted xylan. The XOS-yield (xylobiose – xylopentaose) was evaluated at time intervals in the... (More)
Agricultural by-products are raw materials of importance for increased utilization of renewable biomass. Wheat straw is a raw material of significant production volume and is in this work used for production of xylooligosaccharides (XOS). Extraction of xylan by dilute alkali was followed by hydrolysis using a variant of the alkali-tolerant Bacillus halodurans S7 endoxylanase A mutated at K80R. The xylan yield was on average 56.5 g xylose equivalents per kg dried, ground wheat straw, with 1 arabinose per 12 xylose residues. The K80R variant, which displayed higher specific activity than the wild-type enzyme, was added at a load of 96 U/g extracted xylan. The XOS-yield (xylobiose – xylopentaose) was evaluated at time intervals in the temperature range of 50 to 65 degrees C, at pHs from 7 to 10. The enzyme was optimally active at 60 degrees C up to pH 9. Hydrolysis was completed within 7 h, resulting in 36 % conversion of the xylan to predominantly xylobiose. Xylose content was low (2.4%) despite extended incubation, which is desirable for XOS-production. The XOS-containing hydrolysate was confirmed as a suitable carbon source for the putative probiotic strain Lactobacillus brevis DSM 1269, showing the applicability of the method to obtain prebiotic XOS. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
XOS, Bacillus halodurans, Glycoside hydrolase family 10, GH10, Lactobacillus brevis, arabinoxylan
in
Food and Bioproducts Processing
volume
93
issue
Online 22 November 2014
pages
1 - 10
publisher
Elsevier
external identifiers
  • wos:000349431800001
  • scopus:84922133003
ISSN
1744-3571
DOI
10.1016/j.fbp.2014.11.004
language
English
LU publication?
yes
id
1fd47d13-2c37-4ec8-947f-5f5695b2dec6 (old id 5010926)
date added to LUP
2015-01-28 14:02:40
date last changed
2017-11-12 03:12:08
@article{1fd47d13-2c37-4ec8-947f-5f5695b2dec6,
  abstract     = {Agricultural by-products are raw materials of importance for increased utilization of renewable biomass. Wheat straw is a raw material of significant production volume and is in this work used for production of xylooligosaccharides (XOS). Extraction of xylan by dilute alkali was followed by hydrolysis using a variant of the alkali-tolerant Bacillus halodurans S7 endoxylanase A mutated at K80R. The xylan yield was on average 56.5 g xylose equivalents per kg dried, ground wheat straw, with 1 arabinose per 12 xylose residues. The K80R variant, which displayed higher specific activity than the wild-type enzyme, was added at a load of 96 U/g extracted xylan. The XOS-yield (xylobiose – xylopentaose) was evaluated at time intervals in the temperature range of 50 to 65 degrees C, at pHs from 7 to 10. The enzyme was optimally active at 60 degrees C up to pH 9. Hydrolysis was completed within 7 h, resulting in 36 % conversion of the xylan to predominantly xylobiose. Xylose content was low (2.4%) despite extended incubation, which is desirable for XOS-production. The XOS-containing hydrolysate was confirmed as a suitable carbon source for the putative probiotic strain Lactobacillus brevis DSM 1269, showing the applicability of the method to obtain prebiotic XOS.},
  author       = {Faryar, Reza and Linares-Pastén, Javier and Immerzeel, Peter and Mamo, Gashaw and Andersson, Maria and Stålbrand, Henrik and Mattiasson, Bo and Nordberg Karlsson, Eva},
  issn         = {1744-3571},
  keyword      = {XOS,Bacillus halodurans,Glycoside hydrolase family 10,GH10,Lactobacillus brevis,arabinoxylan},
  language     = {eng},
  number       = {Online 22 November 2014},
  pages        = {1--10},
  publisher    = {Elsevier},
  series       = {Food and Bioproducts Processing},
  title        = {Production of prebiotic xylooligosaccharides from alkaline extracted wheat straw using the K80R-variant of a thermostable alkali-tolerant xylanase},
  url          = {http://dx.doi.org/10.1016/j.fbp.2014.11.004},
  volume       = {93},
  year         = {2015},
}