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Preparation of boronic acid-functionalized cryogels using modular and clickable building blocks for bacterial separation

Zheng, Hongwei LU ; Hajizadeh, Solmaz LU orcid ; Gong, Haiyue LU ; Lin, Hong and Ye, Lei LU orcid (2021) In Journal of Agricultural and Food Chemistry 69(1). p.135-145
Abstract

Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide−alkyne cycloaddition reaction. The boronic acid-functionalized cryogel binds Gram-positive and Gram-negative bacteria through reversible boronate ester bonds, which can be controlled by pH and simple monosaccharides. To increase the capacity of affinity separation, a new approach is used to couple the alkyne-tagged phenylboronic acid to cryogel via an intermediate polymer layer that... (More)

Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide−alkyne cycloaddition reaction. The boronic acid-functionalized cryogel binds Gram-positive and Gram-negative bacteria through reversible boronate ester bonds, which can be controlled by pH and simple monosaccharides. To increase the capacity of affinity separation, a new approach is used to couple the alkyne-tagged phenylboronic acid to cryogel via an intermediate polymer layer that provides multiple immobilization sites. The morphology and chemical composition of the composite cryogel are characterized systematically. The capability of the composite cryogel for the separation of Gram-positive and Gram-negative bacteria is investigated. The binding capacities of the composite cryogel for Escherichia coli and Staphylococcus epidermidis are 2.15 × 109 and 3.36 × 109 cfu/g, respectively. The bacterial binding of the composite cryogel can be controlled by adjusting pH. The results suggest that the composite cryogel may be used as affinity medium for rapid separation and isolation of bacteria from complex samples.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacteria, Bioseparation, Boronate affinity, Composite cryogel, Reversible addition fragmentation chain transfer polymerization
in
Journal of Agricultural and Food Chemistry
volume
69
issue
1
pages
135 - 145
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85099112883
  • pmid:33371673
ISSN
0021-8561
DOI
10.1021/acs.jafc.0c06052
language
English
LU publication?
yes
id
501843c9-d3ad-42ae-ad6d-930bbfdcbd26
date added to LUP
2021-01-20 09:36:27
date last changed
2024-04-18 00:43:40
@article{501843c9-d3ad-42ae-ad6d-930bbfdcbd26,
  abstract     = {{<p>Composite cryogels containing boronic acid ligands are synthesized for effective separation and isolation of bacteria. The large and interconnected pores in cryogels enable fast binding and release of microbial cells. To control bacterial binding, an alkyne-tagged boronic acid ligand is conjugated to azide-functionalized cryogel via the Cu(I)-catalyzed azide−alkyne cycloaddition reaction. The boronic acid-functionalized cryogel binds Gram-positive and Gram-negative bacteria through reversible boronate ester bonds, which can be controlled by pH and simple monosaccharides. To increase the capacity of affinity separation, a new approach is used to couple the alkyne-tagged phenylboronic acid to cryogel via an intermediate polymer layer that provides multiple immobilization sites. The morphology and chemical composition of the composite cryogel are characterized systematically. The capability of the composite cryogel for the separation of Gram-positive and Gram-negative bacteria is investigated. The binding capacities of the composite cryogel for Escherichia coli and Staphylococcus epidermidis are 2.15 × 10<sup>9</sup> and 3.36 × 10<sup>9</sup> cfu/g, respectively. The bacterial binding of the composite cryogel can be controlled by adjusting pH. The results suggest that the composite cryogel may be used as affinity medium for rapid separation and isolation of bacteria from complex samples.</p>}},
  author       = {{Zheng, Hongwei and Hajizadeh, Solmaz and Gong, Haiyue and Lin, Hong and Ye, Lei}},
  issn         = {{0021-8561}},
  keywords     = {{Bacteria; Bioseparation; Boronate affinity; Composite cryogel; Reversible addition fragmentation chain transfer polymerization}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{135--145}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Agricultural and Food Chemistry}},
  title        = {{Preparation of boronic acid-functionalized cryogels using modular and clickable building blocks for bacterial separation}},
  url          = {{http://dx.doi.org/10.1021/acs.jafc.0c06052}},
  doi          = {{10.1021/acs.jafc.0c06052}},
  volume       = {{69}},
  year         = {{2021}},
}