Advanced

Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems.

Isaksson, Jenny; Rasmussen, Magnus LU ; Nilson, Bo LU ; Stadler, Liselott Svensson; Kurland, Siri; Olaison, Lars; Ek, Elisabeth and Herrmann, Björn (2015) In Diagnostic Microbiology and Infectious Disease 81(4). p.240-245
Abstract
Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when... (More)
Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diagnostic Microbiology and Infectious Disease
volume
81
issue
4
pages
240 - 245
publisher
Elsevier
external identifiers
  • pmid:25616316
  • wos:000352117200003
  • scopus:84925156374
ISSN
1879-0070
DOI
10.1016/j.diagmicrobio.2014.12.007
language
English
LU publication?
yes
id
9ff77c46-4d57-45a2-a726-fbb0c0a04286 (old id 5039881)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25616316?dopt=Abstract
date added to LUP
2015-02-05 18:17:37
date last changed
2017-07-02 03:24:43
@article{9ff77c46-4d57-45a2-a726-fbb0c0a04286,
  abstract     = {Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp.},
  author       = {Isaksson, Jenny and Rasmussen, Magnus and Nilson, Bo and Stadler, Liselott Svensson and Kurland, Siri and Olaison, Lars and Ek, Elisabeth and Herrmann, Björn},
  issn         = {1879-0070},
  language     = {eng},
  number       = {4},
  pages        = {240--245},
  publisher    = {Elsevier},
  series       = {Diagnostic Microbiology and Infectious Disease},
  title        = {Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems.},
  url          = {http://dx.doi.org/10.1016/j.diagmicrobio.2014.12.007},
  volume       = {81},
  year         = {2015},
}