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Crosslinked, cryostructured Lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol

Zaushitsyna, Oksana LU ; Dishisha, Tarek LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2017) In Journal of Biotechnology 241. p.22-32
Abstract

Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the... (More)

Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the propanediol-utilization pathway, incorporating glycerol/diol dehydratase, propionaldehyde dehydrogenase, 1,3PDO oxidoreductase, phosphotransacylase, and propionate kinase, for conversion of glycerol to the three chemicals showed that the maximum specific reaction rates were −562.6, 281.4, 62.4 and 50.5 mg/gCDW h for glycerol consumption, and 3HPA (extracellular), 3HP and 1,3PDO production, respectively. Under optimal conditions using monolith operated as continuous plug flow reactor, 19.7 g/L 3HPA was produced as complex with carbohydrazide at a rate of 9.1 g/Lh and a yield of 77 mol%. Using fed-batch operation, 1,3PDO and 3HP were co-produced in equimolar amounts with a yield of 91 mol%. The monoliths embedded in plastic carriers showed high mechanical stability under different modes in a miniaturized plug flow reactor.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell immobilization, Crosslinking, Cryogel monolith, Flux analysis, Glycerol, Lactobacillus reuteri
in
Journal of Biotechnology
volume
241
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:84998610664
  • wos:000392677900004
ISSN
0168-1656
DOI
10.1016/j.jbiotec.2016.11.005
language
English
LU publication?
yes
id
505e2c59-c7f9-47ee-82d8-dc3491d792f0
date added to LUP
2017-03-03 08:56:04
date last changed
2018-01-07 11:53:53
@article{505e2c59-c7f9-47ee-82d8-dc3491d792f0,
  abstract     = {<p>Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the propanediol-utilization pathway, incorporating glycerol/diol dehydratase, propionaldehyde dehydrogenase, 1,3PDO oxidoreductase, phosphotransacylase, and propionate kinase, for conversion of glycerol to the three chemicals showed that the maximum specific reaction rates were −562.6, 281.4, 62.4 and 50.5 mg/g<sub>CDW</sub> h for glycerol consumption, and 3HPA (extracellular), 3HP and 1,3PDO production, respectively. Under optimal conditions using monolith operated as continuous plug flow reactor, 19.7 g/L 3HPA was produced as complex with carbohydrazide at a rate of 9.1 g/Lh and a yield of 77 mol%. Using fed-batch operation, 1,3PDO and 3HP were co-produced in equimolar amounts with a yield of 91 mol%. The monoliths embedded in plastic carriers showed high mechanical stability under different modes in a miniaturized plug flow reactor.</p>},
  author       = {Zaushitsyna, Oksana and Dishisha, Tarek and Hatti-Kaul, Rajni and Mattiasson, Bo},
  issn         = {0168-1656},
  keyword      = {Cell immobilization,Crosslinking,Cryogel monolith,Flux analysis,Glycerol,Lactobacillus reuteri},
  language     = {eng},
  month        = {01},
  pages        = {22--32},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Crosslinked, cryostructured Lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2016.11.005},
  volume       = {241},
  year         = {2017},
}