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Increasing circulating levels of Tenascin C in response to the Wingate Anaerobic test

Ekström, Ola LU ; Ström, Kristoffer LU ; Mir, Bilal Ahmad LU orcid ; Laurila, Esa LU ; Wessman, Ylva LU ; Lehtovirta, Mikko LU ; Eriksson, Karl-Fredrik LU and Hansson, Ola LU orcid (2023) In Clinical Physiology and Functional Imaging 43(4). p.271-277
Abstract

AIM: Tenascin C (TNC) is a large extracellular matrix glycoprotein. It is involved in development and upregulated both during tissue repair and in several pathological conditions, including cardiovascular disease. Extracellular matrix proteins play a role in promoting exercise responses, leading to adaptation, regeneration, and repair. The main goal of this study was to investigate whether a short anaerobic effort leads to increased levels of TNC in serum.

METHODS: Thirty-nine healthy men performed a Wingate test followed by a muscle biopsy. Myoblasts were isolated from the muscle biopsies and differentiated to myotubes ex vivo. TNC RNA was quantified in the biopsies, myotubes and myoblasts using RNA sequencing. Blood samples were... (More)

AIM: Tenascin C (TNC) is a large extracellular matrix glycoprotein. It is involved in development and upregulated both during tissue repair and in several pathological conditions, including cardiovascular disease. Extracellular matrix proteins play a role in promoting exercise responses, leading to adaptation, regeneration, and repair. The main goal of this study was to investigate whether a short anaerobic effort leads to increased levels of TNC in serum.

METHODS: Thirty-nine healthy men performed a Wingate test followed by a muscle biopsy. Myoblasts were isolated from the muscle biopsies and differentiated to myotubes ex vivo. TNC RNA was quantified in the biopsies, myotubes and myoblasts using RNA sequencing. Blood samples were drawn before and 5 min after the Wingate test. Serum TNC levels were measured using ELISA.

RESULTS: After the Wingate test, serum TNC increased on average by 23% [15-33], median [IQR]; P Wilcoxon < 0.0001. This increase is correlated with peak power output and power drop, but not with VO 2max . TNC RNA expression is higher in myoblasts and myotubes compared to skeletal muscle tissue.

CONCLUSION: TNC is secreted systemically as a response to the Wingate anaerobic test in healthy males. The response was positively correlated with peak power and power drop, but not with VO 2max which implicates a relation to mechanical strain and/or blood flow. With higher expression in undifferentiated myoblast cells than muscle tissue, it is likely that TNC plays a role in muscle tissue remodelling in humans. Our findings open for research on how TNC contributes to exercise adaptation. This article is protected by copyright. All rights reserved.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Physiology and Functional Imaging
volume
43
issue
4
pages
271 - 277
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:36943006
  • scopus:85151978501
ISSN
1475-0961
DOI
10.1111/cpf.12816
language
English
LU publication?
yes
additional info
This article is protected by copyright. All rights reserved.
id
509bbf56-ea29-4e85-8e1f-7d7db77fc0a9
date added to LUP
2023-03-23 14:16:08
date last changed
2023-11-22 17:02:43
@article{509bbf56-ea29-4e85-8e1f-7d7db77fc0a9,
  abstract     = {{<p>AIM: Tenascin C (TNC) is a large extracellular matrix glycoprotein. It is involved in development and upregulated both during tissue repair and in several pathological conditions, including cardiovascular disease. Extracellular matrix proteins play a role in promoting exercise responses, leading to adaptation, regeneration, and repair. The main goal of this study was to investigate whether a short anaerobic effort leads to increased levels of TNC in serum.</p><p>METHODS: Thirty-nine healthy men performed a Wingate test followed by a muscle biopsy. Myoblasts were isolated from the muscle biopsies and differentiated to myotubes ex vivo. TNC RNA was quantified in the biopsies, myotubes and myoblasts using RNA sequencing. Blood samples were drawn before and 5 min after the Wingate test. Serum TNC levels were measured using ELISA.</p><p>RESULTS: After the Wingate test, serum TNC increased on average by 23% [15-33], median [IQR]; P Wilcoxon &lt; 0.0001. This increase is correlated with peak power output and power drop, but not with VO  2max . TNC RNA expression is higher in myoblasts and myotubes compared to skeletal muscle tissue. </p><p>CONCLUSION: TNC is secreted systemically as a response to the Wingate anaerobic test in healthy males. The response was positively correlated with peak power and power drop, but not with VO 2max which implicates a relation to mechanical strain and/or blood flow. With higher expression in undifferentiated myoblast cells than muscle tissue, it is likely that TNC plays a role in muscle tissue remodelling in humans. Our findings open for research on how TNC contributes to exercise adaptation. This article is protected by copyright. All rights reserved. </p>}},
  author       = {{Ekström, Ola and Ström, Kristoffer and Mir, Bilal Ahmad and Laurila, Esa and Wessman, Ylva and Lehtovirta, Mikko and Eriksson, Karl-Fredrik and Hansson, Ola}},
  issn         = {{1475-0961}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{4}},
  pages        = {{271--277}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Clinical Physiology and Functional Imaging}},
  title        = {{Increasing circulating levels of Tenascin C in response to the Wingate Anaerobic test}},
  url          = {{http://dx.doi.org/10.1111/cpf.12816}},
  doi          = {{10.1111/cpf.12816}},
  volume       = {{43}},
  year         = {{2023}},
}