Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
(2022) In ACS Pharmacology and Translational Science 5(3). p.141-148- Abstract
COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that... (More)
COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that observed in COVID-19. Therefore, the model offers utility for analyses of the pathophysiological features of COVID-19 and the development of new treatments.
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- author
- Puthia, Manoj LU ; Tanner, Lloyd LU ; Petruk, Ganna LU and Schmidtchen, Artur LU
- organization
- publishing date
- 2022-03
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ARDS, COVID-19 in vivo models, LPS, SARS-CoV-2 spike (S) protein, TCP-25
- in
- ACS Pharmacology and Translational Science
- volume
- 5
- issue
- 3
- pages
- 8 pages
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:35774232
- scopus:85124131709
- DOI
- 10.1021/acsptsci.1c00219
- language
- English
- LU publication?
- yes
- id
- 50ecf9d2-4e5c-4fe9-befa-f7a7e16cf218
- date added to LUP
- 2022-04-05 15:31:48
- date last changed
- 2024-09-10 15:12:07
@article{50ecf9d2-4e5c-4fe9-befa-f7a7e16cf218, abstract = {{<p>COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that observed in COVID-19. Therefore, the model offers utility for analyses of the pathophysiological features of COVID-19 and the development of new treatments.</p>}}, author = {{Puthia, Manoj and Tanner, Lloyd and Petruk, Ganna and Schmidtchen, Artur}}, keywords = {{ARDS; COVID-19 in vivo models; LPS; SARS-CoV-2 spike (S) protein; TCP-25}}, language = {{eng}}, number = {{3}}, pages = {{141--148}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Pharmacology and Translational Science}}, title = {{Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin}}, url = {{http://dx.doi.org/10.1021/acsptsci.1c00219}}, doi = {{10.1021/acsptsci.1c00219}}, volume = {{5}}, year = {{2022}}, }