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Is label-free LC-MS/MS ready for biomarker discovery?

Sandin, Marianne LU ; Chawade, Aakash LU and Levander, Fredrik LU (2015) In Proteomics Clinical Applications 9(3-4). p.289-294
Abstract
Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of... (More)
Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future. This article is protected by copyright. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proteomics Clinical Applications
volume
9
issue
3-4
pages
289 - 294
publisher
John Wiley & Sons
external identifiers
  • pmid:25656266
  • wos:000353291000005
  • scopus:84928062047
ISSN
1862-8354
DOI
10.1002/prca.201400202
language
English
LU publication?
yes
id
ea083c77-821c-48fc-a681-ceb66c0a591e (old id 5145330)
date added to LUP
2015-03-09 14:03:44
date last changed
2017-04-09 03:12:23
@misc{ea083c77-821c-48fc-a681-ceb66c0a591e,
  abstract     = {Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future. This article is protected by copyright. All rights reserved.},
  author       = {Sandin, Marianne and Chawade, Aakash and Levander, Fredrik},
  issn         = {1862-8354},
  language     = {eng},
  number       = {3-4},
  pages        = {289--294},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics Clinical Applications},
  title        = {Is label-free LC-MS/MS ready for biomarker discovery?},
  url          = {http://dx.doi.org/10.1002/prca.201400202},
  volume       = {9},
  year         = {2015},
}