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Little difference between minimum inhibitory concentrations of Mycobacterium tuberculosis wild-type organisms determined with BACTEC MGIT 960 and Middlebrook 7H10.

Sturegård, Erik LU ; Ängeby, K A; Werngren, J; Juréen, P; Kronvall, G; Giske, C G; Kahlmeter, G and Schön, T (2015) In Clinical Microbiology and Infection 21(2). p.5-148
Abstract
The MIC wild-type (WT) distribution for Mycobacterium tuberculosis in BACTEC 960 MGIT is not defined, which may result in poor reproducibility for drug susceptibility testing (DST), as several DST methods with different breakpoints are in use. In a comparison between MGIT and Middlebrook 7H10 medium of seven first- and second-line drugs, including 133 MIC determinations of 15 WT isolates, we found an agreement of 91.7% within ± one MIC dilution step. The results confirm the agreement in MIC testing between 7H10 and MGIT and indicate that breakpoints could be harmonized in order to avoid misclassification.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Microbiology and Infection
volume
21
issue
2
pages
5 - 148
publisher
Wiley-Blackwell
external identifiers
  • pmid:25640156
  • scopus:84923013605
ISSN
1469-0691
DOI
10.1016/j.cmi.2014.08.021
language
English
LU publication?
yes
id
1f6a72d6-9a71-47d6-a4d0-0ec5cc4e55d1 (old id 5145714)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25640156?dopt=Abstract
date added to LUP
2015-03-03 16:39:23
date last changed
2017-01-01 07:38:16
@article{1f6a72d6-9a71-47d6-a4d0-0ec5cc4e55d1,
  abstract     = {The MIC wild-type (WT) distribution for Mycobacterium tuberculosis in BACTEC 960 MGIT is not defined, which may result in poor reproducibility for drug susceptibility testing (DST), as several DST methods with different breakpoints are in use. In a comparison between MGIT and Middlebrook 7H10 medium of seven first- and second-line drugs, including 133 MIC determinations of 15 WT isolates, we found an agreement of 91.7% within ± one MIC dilution step. The results confirm the agreement in MIC testing between 7H10 and MGIT and indicate that breakpoints could be harmonized in order to avoid misclassification.},
  author       = {Sturegård, Erik and Ängeby, K A and Werngren, J and Juréen, P and Kronvall, G and Giske, C G and Kahlmeter, G and Schön, T},
  issn         = {1469-0691},
  language     = {eng},
  number       = {2},
  pages        = {5--148},
  publisher    = {Wiley-Blackwell},
  series       = {Clinical Microbiology and Infection},
  title        = {Little difference between minimum inhibitory concentrations of Mycobacterium tuberculosis wild-type organisms determined with BACTEC MGIT 960 and Middlebrook 7H10.},
  url          = {http://dx.doi.org/10.1016/j.cmi.2014.08.021},
  volume       = {21},
  year         = {2015},
}