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Effective and highly selective lipase-mediated synthesis of 2-monoolein and 1,2-diolein in a two-phase system

Mangas, Juan LU ; Serrano-Arnaldos, Mar and Adlercreutz, Patrick LU (2015) In Journal of Molecular Catalysis B: Enzymatic 112. p.9-14
Abstract
The possibility of using 1,3-regioselective lipases for the synthesis of 2-monoolein and 1,2-diolein by ethanolysis of triolein was evaluated. The reactions were carried out in aqueous/organic two-phase systems, achieving high reaction rates due to the interfacial activation of the lipases. Different lipases and reaction conditions were screened. Commercially available lipases were used without purification or immobilisation. After preliminary tests, response surface analysis was used to optimise the reaction conditions for the most promising enzymes. Rhizopus oryzae lipase was the best one for the synthesis of 2-monoolein, giving a 76% yield with an isomer composition of 99:1. Pseudomonas fluorescens lipase was the most favourable one for... (More)
The possibility of using 1,3-regioselective lipases for the synthesis of 2-monoolein and 1,2-diolein by ethanolysis of triolein was evaluated. The reactions were carried out in aqueous/organic two-phase systems, achieving high reaction rates due to the interfacial activation of the lipases. Different lipases and reaction conditions were screened. Commercially available lipases were used without purification or immobilisation. After preliminary tests, response surface analysis was used to optimise the reaction conditions for the most promising enzymes. Rhizopus oryzae lipase was the best one for the synthesis of 2-monoolein, giving a 76% yield with an isomer composition of 99:1. Pseudomonas fluorescens lipase was the most favourable one for the synthesis of 1,2-diolein, giving a 58% yield and an isomer composition of 93:7. (C) 2014 The Authors. Published by Elsevier B.V. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
2-Dioolein, 1, 2-Monoolein, Two-phase system, Lipase, Ethanolysis
in
Journal of Molecular Catalysis B: Enzymatic
volume
112
pages
9 - 14
publisher
Elsevier
external identifiers
  • wos:000349592700002
  • scopus:84927708879
ISSN
1873-3158
DOI
10.1016/j.molcatb.2014.11.014
language
English
LU publication?
yes
id
98fa8639-2949-45ca-a04b-a9c895a99b0e (old id 5160254)
date added to LUP
2015-03-25 09:04:36
date last changed
2017-09-17 04:19:28
@article{98fa8639-2949-45ca-a04b-a9c895a99b0e,
  abstract     = {The possibility of using 1,3-regioselective lipases for the synthesis of 2-monoolein and 1,2-diolein by ethanolysis of triolein was evaluated. The reactions were carried out in aqueous/organic two-phase systems, achieving high reaction rates due to the interfacial activation of the lipases. Different lipases and reaction conditions were screened. Commercially available lipases were used without purification or immobilisation. After preliminary tests, response surface analysis was used to optimise the reaction conditions for the most promising enzymes. Rhizopus oryzae lipase was the best one for the synthesis of 2-monoolein, giving a 76% yield with an isomer composition of 99:1. Pseudomonas fluorescens lipase was the most favourable one for the synthesis of 1,2-diolein, giving a 58% yield and an isomer composition of 93:7. (C) 2014 The Authors. Published by Elsevier B.V.},
  author       = {Mangas, Juan and Serrano-Arnaldos, Mar and Adlercreutz, Patrick},
  issn         = {1873-3158},
  keyword      = {2-Dioolein,1,2-Monoolein,Two-phase system,Lipase,Ethanolysis},
  language     = {eng},
  pages        = {9--14},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Catalysis B: Enzymatic},
  title        = {Effective and highly selective lipase-mediated synthesis of 2-monoolein and 1,2-diolein in a two-phase system},
  url          = {http://dx.doi.org/10.1016/j.molcatb.2014.11.014},
  volume       = {112},
  year         = {2015},
}