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Autophagy in the posterior interosseous nerve of patients with type 1 and type 2 diabetes mellitus: an ultrastructural study

Osman, Ayman A. M.; Dahlin, Lars LU ; Thomsen, Niels LU and Mohseni, Simin (2015) In Diabetologia 58(3). p.625-632
Abstract
We addressed the question of whether the autophagy pathway occurs in human peripheral nerves and whether this pathway is associated with peripheral neuropathy in diabetes mellitus. By using electron microscopy, we evaluated the presence of autophagy-related structures and neuropathy in the posterior interosseous nerve of patients who had undergone carpal tunnel release and had type 1 or type 2 diabetes mellitus, and in patients with no diabetes (controls). Autophagy-related ultrastructures were observed in the samples taken from all patients of the three groups. The number of autophagy-associated structures was significantly higher (p < 0.05) in the nerves of patients with type 1 than type 2 diabetes. Qualitative and quantitative... (More)
We addressed the question of whether the autophagy pathway occurs in human peripheral nerves and whether this pathway is associated with peripheral neuropathy in diabetes mellitus. By using electron microscopy, we evaluated the presence of autophagy-related structures and neuropathy in the posterior interosseous nerve of patients who had undergone carpal tunnel release and had type 1 or type 2 diabetes mellitus, and in patients with no diabetes (controls). Autophagy-related ultrastructures were observed in the samples taken from all patients of the three groups. The number of autophagy-associated structures was significantly higher (p < 0.05) in the nerves of patients with type 1 than type 2 diabetes. Qualitative and quantitative evaluations of fascicle area, diameter of myelinated and unmyelinated nerve fibres, the density of myelinated and unmyelinated fibres and the g-ratio of myelinated fibres were performed. We found degeneration and regeneration of a few myelinated axons in controls, and a well-developed neuropathy with the loss of large myelinated axons and the presence of many small ones in patients with diabetes. The pathology in type 1 diabetes was more extensive than in type 2 diabetes. The results of this study show that the human peripheral nerves have access to the autophagy machinery, and this pathway may be regulated differently in type 1 and type 2 diabetes; insulin, presence of extensive neuropathy, and/or other factors such as duration of diabetes and HbA(1c) level may underlie this differential regulation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autophagy, Diabetes, Electron microscopy, Human, Neuropathy, Peripheral, nerve
in
Diabetologia
volume
58
issue
3
pages
625 - 632
publisher
Springer Verlag
external identifiers
  • wos:000349244100024
  • scopus:84925499821
ISSN
1432-0428
DOI
10.1007/s00125-014-3477-4
language
English
LU publication?
yes
id
fe2de7ab-edce-4872-b892-c6b0c089be47 (old id 5172975)
date added to LUP
2015-04-01 07:38:42
date last changed
2017-08-27 03:20:27
@article{fe2de7ab-edce-4872-b892-c6b0c089be47,
  abstract     = {We addressed the question of whether the autophagy pathway occurs in human peripheral nerves and whether this pathway is associated with peripheral neuropathy in diabetes mellitus. By using electron microscopy, we evaluated the presence of autophagy-related structures and neuropathy in the posterior interosseous nerve of patients who had undergone carpal tunnel release and had type 1 or type 2 diabetes mellitus, and in patients with no diabetes (controls). Autophagy-related ultrastructures were observed in the samples taken from all patients of the three groups. The number of autophagy-associated structures was significantly higher (p &lt; 0.05) in the nerves of patients with type 1 than type 2 diabetes. Qualitative and quantitative evaluations of fascicle area, diameter of myelinated and unmyelinated nerve fibres, the density of myelinated and unmyelinated fibres and the g-ratio of myelinated fibres were performed. We found degeneration and regeneration of a few myelinated axons in controls, and a well-developed neuropathy with the loss of large myelinated axons and the presence of many small ones in patients with diabetes. The pathology in type 1 diabetes was more extensive than in type 2 diabetes. The results of this study show that the human peripheral nerves have access to the autophagy machinery, and this pathway may be regulated differently in type 1 and type 2 diabetes; insulin, presence of extensive neuropathy, and/or other factors such as duration of diabetes and HbA(1c) level may underlie this differential regulation.},
  author       = {Osman, Ayman A. M. and Dahlin, Lars and Thomsen, Niels and Mohseni, Simin},
  issn         = {1432-0428},
  keyword      = {Autophagy,Diabetes,Electron microscopy,Human,Neuropathy,Peripheral,nerve},
  language     = {eng},
  number       = {3},
  pages        = {625--632},
  publisher    = {Springer Verlag},
  series       = {Diabetologia},
  title        = {Autophagy in the posterior interosseous nerve of patients with type 1 and type 2 diabetes mellitus: an ultrastructural study},
  url          = {http://dx.doi.org/10.1007/s00125-014-3477-4},
  volume       = {58},
  year         = {2015},
}