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Stabilization of Adsorbed Enzymes Used as Biocatalysts in Organic Solvents

Wehtje, Ernst LU ; Adlercreutz, Patrick LU orcid and Mattiasson, Bo LU (1992) In Progress in Biotechnology 8(C). p.377-382
Abstract

Enzymes immobilized by deposition onto a solid support at low enzyme loadings were shown to be stabilized by additives, such as proteins and PEG. The additive protects the enzyme from deactivation during immobilization. The amount of additive required to obtain full stabilization was dependent on the type of support material used and corresponded approximately to a monolayer coverage of the additive on the surface of the support. The stabilizing effect of the additive was less pronounced at high enzyme loadings.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Progress in Biotechnology
series title
Progress in Biotechnology
volume
8
issue
C
edition
C
pages
6 pages
external identifiers
  • scopus:21344434975
ISSN
0921-0423
DOI
10.1016/B978-0-444-89046-7.50058-8
language
English
LU publication?
yes
id
51c07354-d38b-4b83-98fb-cccada3a31dd
date added to LUP
2019-06-22 09:25:13
date last changed
2025-04-04 14:36:00
@inbook{51c07354-d38b-4b83-98fb-cccada3a31dd,
  abstract     = {{<p>Enzymes immobilized by deposition onto a solid support at low enzyme loadings were shown to be stabilized by additives, such as proteins and PEG. The additive protects the enzyme from deactivation during immobilization. The amount of additive required to obtain full stabilization was dependent on the type of support material used and corresponded approximately to a monolayer coverage of the additive on the surface of the support. The stabilizing effect of the additive was less pronounced at high enzyme loadings.</p>}},
  author       = {{Wehtje, Ernst and Adlercreutz, Patrick and Mattiasson, Bo}},
  booktitle    = {{Progress in Biotechnology}},
  issn         = {{0921-0423}},
  language     = {{eng}},
  number       = {{C}},
  pages        = {{377--382}},
  series       = {{Progress in Biotechnology}},
  title        = {{Stabilization of Adsorbed Enzymes Used as Biocatalysts in Organic Solvents}},
  url          = {{http://dx.doi.org/10.1016/B978-0-444-89046-7.50058-8}},
  doi          = {{10.1016/B978-0-444-89046-7.50058-8}},
  volume       = {{8}},
  year         = {{1992}},
}