A mathematical model of the euglycemic hyperinsulinemic clamp
(2005) In Theoretical Biology Medical Modelling 2(44).- Abstract
- Background
The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.
Results
From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of... (More) - Background
The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.
Results
From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of obese subjects was assessed analytically. Obese subjects had significantly less effective suppression of hepatic glucose output and higher pancreatic insulin secretion than lean subjects. Tissue insulin resistance appeared to be higher in the obese group, but this difference did not reach statistical significance.
Conclusion
The use of a mathematical model allows a greater amount of information to be recovered from clamp data, making it easier to understand the components of insulin resistance in obese vs. normal subjects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4216019
- author
- Picchini, Umberto LU ; De Gaetano, Andrea ; Panunzi, Simona ; Ditlevsen, Susanne and Mingrone, Geltrude
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Theoretical Biology Medical Modelling
- volume
- 2
- issue
- 44
- publisher
- BioMed Central (BMC)
- external identifiers
-
- scopus:28444441579
- pmid:16269082
- ISSN
- 1742-4682
- DOI
- 10.1186/1742-4682-2-44
- language
- English
- LU publication?
- no
- id
- 52308f78-81ed-4593-9999-3aefc7d69646 (old id 4216019)
- date added to LUP
- 2016-04-01 16:56:19
- date last changed
- 2022-01-28 23:12:05
@article{52308f78-81ed-4593-9999-3aefc7d69646,
abstract = {{Background<br/><br>
The Euglycemic Hyperinsulinemic Clamp (EHC) is the most widely used experimental procedure for the determination of insulin sensitivity, and in its usual form the patient is followed under insulinization for two hours. In the present study, sixteen subjects with BMI between 18.5 and 63.6 kg/m2 were studied by long-duration (five hours) EHC.<br/><br>
<br/><br>
Results<br/><br>
From the results of this series and from similar reports in the literature it is clear that, in obese subjects, glucose uptake rates continue to increase if the clamp procedure is prolonged beyond the customary 2 hours. A mathematical model of the EHC, incorporating delays, was fitted to the recorded data, and the insulin resistance behaviour of obese subjects was assessed analytically. Obese subjects had significantly less effective suppression of hepatic glucose output and higher pancreatic insulin secretion than lean subjects. Tissue insulin resistance appeared to be higher in the obese group, but this difference did not reach statistical significance.<br/><br>
<br/><br>
Conclusion<br/><br>
The use of a mathematical model allows a greater amount of information to be recovered from clamp data, making it easier to understand the components of insulin resistance in obese vs. normal subjects.}},
author = {{Picchini, Umberto and De Gaetano, Andrea and Panunzi, Simona and Ditlevsen, Susanne and Mingrone, Geltrude}},
issn = {{1742-4682}},
language = {{eng}},
number = {{44}},
publisher = {{BioMed Central (BMC)}},
series = {{Theoretical Biology Medical Modelling}},
title = {{A mathematical model of the euglycemic hyperinsulinemic clamp}},
url = {{https://lup.lub.lu.se/search/files/4824199/4216020}},
doi = {{10.1186/1742-4682-2-44}},
volume = {{2}},
year = {{2005}},
}