Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae
(2023) In Methods in molecular biology (Clifton, N.J.) p.131-146- Abstract
Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. In this chapter we describe how the protease IdeS from Streptococcus pyogenes can be expressed in S. cerevisiae. The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. The protease presence was confirmed with a... (More)
Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. In this chapter we describe how the protease IdeS from Streptococcus pyogenes can be expressed in S. cerevisiae. The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. The protease presence was confirmed with a Western blot assay and activity was measured with an in vitro assay. To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein.
(Less)
- author
- Lindh, Tova LU ; Collin, Mattias LU ; Lood, Rolf LU and Carlquist, Magnus LU
- organization
- publishing date
- 2023
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Saccharomyces cerevisiae/genetics, Bacterial Proteins/metabolism, Recombinant Fusion Proteins/genetics, Fluorescence, Peptide Hydrolases/metabolism
- host publication
- Bacterial pathogenesis : Methods and protocols - Methods and protocols
- series title
- Methods in molecular biology (Clifton, N.J.)
- editor
- Nordenfelt, Pontus and Collin, Mattias
- edition
- 2nd
- pages
- 131 - 146
- publisher
- Humana Press
- external identifiers
-
- pmid:37258965
- scopus:85160781455
- ISSN
- 1940-6029
- ISBN
- 978-1-0716-3243-7
- DOI
- 10.1007/978-1-0716-3243-7_9
- language
- English
- LU publication?
- yes
- id
- 524a6104-c032-48a9-a4b1-bcaac8268395
- date added to LUP
- 2023-06-07 09:35:49
- date last changed
- 2024-09-21 12:53:13
@inbook{524a6104-c032-48a9-a4b1-bcaac8268395, abstract = {{<p>Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. In this chapter we describe how the protease IdeS from Streptococcus pyogenes can be expressed in S. cerevisiae. The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. The protease presence was confirmed with a Western blot assay and activity was measured with an in vitro assay. To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein.</p>}}, author = {{Lindh, Tova and Collin, Mattias and Lood, Rolf and Carlquist, Magnus}}, booktitle = {{Bacterial pathogenesis : Methods and protocols}}, editor = {{Nordenfelt, Pontus and Collin, Mattias}}, isbn = {{978-1-0716-3243-7}}, issn = {{1940-6029}}, keywords = {{Saccharomyces cerevisiae/genetics; Bacterial Proteins/metabolism; Recombinant Fusion Proteins/genetics; Fluorescence; Peptide Hydrolases/metabolism}}, language = {{eng}}, pages = {{131--146}}, publisher = {{Humana Press}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae}}, url = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_9}}, doi = {{10.1007/978-1-0716-3243-7_9}}, year = {{2023}}, }