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Inhibitory effects on osteoblast differentiation in vitro by the polychlorinated biphenyl mixture Aroclor 1254 are mainly associated with the dioxin-like constituents.

Herlin, Maria LU ; Öberg, Mattias; Ringblom, Joakim; Joseph, Bertrand; Korkalainen, Merja; Viluksela, Matti; Heimeier, Rachel A and Håkansson, Helen (2015) In Toxicology in Vitro 29(5). p.876-883
Abstract
The polychlorinated biphenyl (PCB) mixture Aroclor 1254 alters bone tissue properties. However, the mechanisms responsible for the observed effects have not yet been clarified. This study compared the effect of Aroclor 1254 on the expression of osteoblast differentiation markers in MC3T3-E1 cells with the corresponding effect of the dioxin reference compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and two PCB congeners belonging to the category of non-dioxin-like PCBs. The aim of the study was to quantify the relative influence of dioxin-like and non-dioxin-like PCB-components on osteoblast differentiation. Expression of marker genes for AhR activity and osteoblast differentiation were analyzed, and relative potency (REP) values were... (More)
The polychlorinated biphenyl (PCB) mixture Aroclor 1254 alters bone tissue properties. However, the mechanisms responsible for the observed effects have not yet been clarified. This study compared the effect of Aroclor 1254 on the expression of osteoblast differentiation markers in MC3T3-E1 cells with the corresponding effect of the dioxin reference compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and two PCB congeners belonging to the category of non-dioxin-like PCBs. The aim of the study was to quantify the relative influence of dioxin-like and non-dioxin-like PCB-components on osteoblast differentiation. Expression of marker genes for AhR activity and osteoblast differentiation were analyzed, and relative potency (REP) values were derived from Benchmark concentration-effect curves. Expression of alkaline phosphatase and osteocalcin were decreased by both Aroclor 1254 and TCDD exposure, while the PCB-congeners PCB19 and PCB52 slightly induced the expression. The relative potency of Aroclor 1254 for inhibitory effects on osteoblast differentiation marker genes was within the expected range as estimated from the chemical composition of Aroclor 1254. These results are consistent with previously observed bone modulations following in vivo exposure to Aroclor 1254 and TCDD, and demonstrate that the inhibitory effects of Aroclor 1254 on osteoblast differentiation by the dioxin-like constituents are over-riding the contribution of non-dioxin-like PCBs. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Toxicology in Vitro
volume
29
issue
5
pages
876 - 883
publisher
Elsevier
external identifiers
  • pmid:25795401
  • wos:000356981500007
  • scopus:84926310737
ISSN
1879-3177
DOI
10.1016/j.tiv.2015.03.006
language
English
LU publication?
yes
id
137ec3e7-c4f6-42de-b568-094436102c31 (old id 5257937)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25795401?dopt=Abstract
date added to LUP
2015-04-05 14:01:51
date last changed
2017-09-03 03:16:25
@article{137ec3e7-c4f6-42de-b568-094436102c31,
  abstract     = {The polychlorinated biphenyl (PCB) mixture Aroclor 1254 alters bone tissue properties. However, the mechanisms responsible for the observed effects have not yet been clarified. This study compared the effect of Aroclor 1254 on the expression of osteoblast differentiation markers in MC3T3-E1 cells with the corresponding effect of the dioxin reference compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and two PCB congeners belonging to the category of non-dioxin-like PCBs. The aim of the study was to quantify the relative influence of dioxin-like and non-dioxin-like PCB-components on osteoblast differentiation. Expression of marker genes for AhR activity and osteoblast differentiation were analyzed, and relative potency (REP) values were derived from Benchmark concentration-effect curves. Expression of alkaline phosphatase and osteocalcin were decreased by both Aroclor 1254 and TCDD exposure, while the PCB-congeners PCB19 and PCB52 slightly induced the expression. The relative potency of Aroclor 1254 for inhibitory effects on osteoblast differentiation marker genes was within the expected range as estimated from the chemical composition of Aroclor 1254. These results are consistent with previously observed bone modulations following in vivo exposure to Aroclor 1254 and TCDD, and demonstrate that the inhibitory effects of Aroclor 1254 on osteoblast differentiation by the dioxin-like constituents are over-riding the contribution of non-dioxin-like PCBs.},
  author       = {Herlin, Maria and Öberg, Mattias and Ringblom, Joakim and Joseph, Bertrand and Korkalainen, Merja and Viluksela, Matti and Heimeier, Rachel A and Håkansson, Helen},
  issn         = {1879-3177},
  language     = {eng},
  number       = {5},
  pages        = {876--883},
  publisher    = {Elsevier},
  series       = {Toxicology in Vitro},
  title        = {Inhibitory effects on osteoblast differentiation in vitro by the polychlorinated biphenyl mixture Aroclor 1254 are mainly associated with the dioxin-like constituents.},
  url          = {http://dx.doi.org/10.1016/j.tiv.2015.03.006},
  volume       = {29},
  year         = {2015},
}