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Antibodies reactive to cleaved sites in complement proteins enable highly specific measurement of soluble markers of complement activation.

Blom, Anna LU ; Österborg, Anders; Mollnes, Tom E and Okroj, Marcin LU (2015) In Molecular Immunology 66(2). p.164-170
Abstract
An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies... (More)
An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies reactive to C4d and C5b fragments enabled us to set up highly specific sandwich ELISAs, which ensured trustful measurements without false positive readouts characteristic for some of the widely used assays. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Immunology
volume
66
issue
2
pages
164 - 170
publisher
Pergamon
external identifiers
  • pmid:25795308
  • wos:000356995900007
  • scopus:84924958505
ISSN
1872-9142
DOI
10.1016/j.molimm.2015.02.029
language
English
LU publication?
yes
id
9e56005f-5133-4094-ae55-eb490b5de970 (old id 5257948)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25795308?dopt=Abstract
date added to LUP
2015-04-05 14:03:12
date last changed
2017-09-03 03:12:19
@article{9e56005f-5133-4094-ae55-eb490b5de970,
  abstract     = {An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies reactive to C4d and C5b fragments enabled us to set up highly specific sandwich ELISAs, which ensured trustful measurements without false positive readouts characteristic for some of the widely used assays.},
  author       = {Blom, Anna and Österborg, Anders and Mollnes, Tom E and Okroj, Marcin},
  issn         = {1872-9142},
  language     = {eng},
  number       = {2},
  pages        = {164--170},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Antibodies reactive to cleaved sites in complement proteins enable highly specific measurement of soluble markers of complement activation.},
  url          = {http://dx.doi.org/10.1016/j.molimm.2015.02.029},
  volume       = {66},
  year         = {2015},
}